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Using qPCR to Identify Potential Effects of Thermal Conditions during Embryogenesis on Mitochondrial DNA Copy Number in Juvenile Brown Trout Salmo trutta
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Environmental and Life Sciences (from 2013).ORCID iD: 0000-0002-3813-9548
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Environmental and Life Sciences (from 2013).
Norwegian Institute for Nature Research, Norway.
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Environmental and Life Sciences (from 2013).ORCID iD: 0000-0002-3191-7140
2024 (English)In: Fishes, E-ISSN 2410-3888, Vol. 9, no 4, article id 142Article in journal (Refereed) Published
Abstract [en]

Changes in the number, structure, and function of mitochondria during the early life stages of animals can play an important role for an organism's metabolic rate, growth, and health. Previous studies have shown that juvenile brown trout (Salmo trutta) subjected to elevated temperatures during the embryonic stage respond phenotypically with a reduced metabolic rate. The aim of this study was to explore if embryonic temperature affects the mitochondria content of young brown trout and as such explains the previously found differences in metabolic rates. Here, we optimize a quantitative PCR (qPCR) method for the mitochondria cytochrome c oxidase subunit I gene, and then use the method as a proxy for mitochondrial DNA content. We hypothesize that young trout subjected to elevated temperatures during the embryonic stage respond phenotypically with a reduced mitochondrial DNA content. To test this hypothesis, we subjected brown trout to either control ambient (4.4 +/- 1.5 degrees C) or elevated temperatures (7.1 +/- 0.6 degrees C) during embryogenesis. Subsequently, we extracted DNA from liver and white muscle tissue of juvenile brown trout from the two different incubation temperature treatments and successively optimized qPCR for mitochondrial DNA. We found that the amount of mitochondria DNA in liver tissue was 18 times higher than in white muscle tissue, but there was no significant difference in mitochondria content in liver or muscle tissue between brown trout exposed to elevated and ambient control temperatures during embryogenesis. We conclude that reduced metabolic rate is not likely associated with mitochondria DNA content. We also suggest that qPCR is a simple and cost-effective method to quantify mitochondria DNA in frozen and partly degraded tissue from different treatment groups and a useful proxy for identification of differences in mitochondria number.

Place, publisher, year, edition, pages
MDPI, 2024. Vol. 9, no 4, article id 142
Keywords [en]
COI gene, mitochondria, Salmo trutta, climate change, quantitative PCR
National Category
Ecology
Research subject
Biology
Identifiers
URN: urn:nbn:se:kau:diva-99858DOI: 10.3390/fishes9040142ISI: 001211346600001Scopus ID: 2-s2.0-85191563994OAI: oai:DiVA.org:kau-99858DiVA, id: diva2:1865005
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Karlstad UniversityAvailable from: 2024-06-04 Created: 2024-06-04 Last updated: 2024-06-13Bibliographically approved

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Erlandsson, AnnGreenberg, Larry

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2526272829303128 of 42
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