Turning to the experiences of Danish sperm donors, I argue that the conception of sperm donation as selfish pleasure and commodified practice offers only a limited understanding of what it means to provide semen samples for reproductive donation. Framing masturbation at sperm banks only in light of selfishness, since it involves sexual pleasure and monetary compensation, ignores the intricate interplay between biomedical regulation, modes of production, and men’s gender performativity. As the accounts by Danish men of masturbation at sperm banks show, reproductive donation as both a moral and economic endeavor relies on the control of male masturbation. Rather than only being a selfish undertaking, providing semen samples becomes understandable as a site of biopolitics altering men’s gender identity. The global supply of donor semen is enabled by regulating the affective spaces of male masturbation in which men remake their masculine self-images.