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  • 1.
    Abdel-Rehim, Mohamed
    et al.
    Karlstad University, Division for Chemistry.
    Altun, Zeki
    Karlstad University, Division for Chemistry.
    Blomberg, Lars
    Karlstad University, Division for Chemistry.
    Microextraction in packed syringe (MEPS) for liquid and gas chromatographic applications: Part II - Determination of ropivacaine and its metabolites in human plasma samples using MEPS with liquid chromatography/tandem mass spectrometry2004In: Journal of Mass Spectrometry, ISSN 1076-5174, E-ISSN 1096-9888, Vol. 39, no 12, p. 1488-1493Article in journal (Refereed)
  • 2.
    Roepstorff, P.
    et al.
    Department of Molecular Biology, Odense University, Denmark.
    Højrup, P.
    Department of Molecular Biology, Odense University, Denmark.
    Sundqvist, B.U.R.
    Tandem Accelerator Laboratory, Uppsala University.
    Jonsson, Gunnar
    Tandem Accelerator Laboratory, Uppsala University.
    Håkansson, P.
    Tandem Accelerator Laboratory, Uppsala University.
    Andersson, S. O.
    Institute for Biological Chemistry A, University of Copenhagen, Denmark.
    Johansson, K. E.
    Mycoplasma Laboratory, The National Veterinary Institute, Uppsala.
    Application of Plasma Desorption Mass Spectrometry to Molecular Weight Determination of Structural Protein from Insect Cuticle1986In: Journal of Mass Spectrometry, ISSN 1076-5174, E-ISSN 1096-9888, Vol. 13, no 12, p. 689-691Article in journal (Refereed)
    Abstract [en]

    During sequence determination of a structural protein from the cuticle of the migratory locust, Locusta migratoria, a discrepancy was found between the molecular weight calculated from the sequence data (15 323) and that estimated by SDS-gel electrophoresis (21 600). The protein contains several repeated sequences, and the discrepancy might indicate that part of the sequence was missing or that the protein contained a large prosthetic group. The molecular weight of the protein was determined by 127I-plasma desorption mass spectrometry to be 15 329±50 which confirms the sequence data and shows the molecular weight determination based on SDS-electrophoresis to be too high.

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