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  • 1.
    Alriksson, Björn
    et al.
    Karlstad University, Division for Chemistry.
    Horváth, Ilona Sárvári
    Karlstad University, Division for Chemistry.
    Sjöde, Anders
    Karlstad University, Division for Chemistry.
    Nilvebrant, Nils-Olof
    Karlstad University, Division for Chemistry.
    Jönsson, Leif J
    Karlstad University, Division for Chemistry.
    Ammonium hydroxide detoxification of spruce acid hydrolysates.2005In: Applied Biochemistry and Biotechnology, ISSN 0273-2289, E-ISSN 1559-0291, Vol. 121-124, p. 911-22Article in journal (Refereed)
    Abstract [en]

    When dilute-acid hydrolysates from spruce are fermented to produce ethanol, detoxification is required to make the hydrolysates fermentable at reasonable rates. Treatment with alkali, usually by overliming, is one of the most efficient approaches. Several nutrients, such as ammonium and phosphate, are added to the hydrolysates prior to fermentation. We investigated the use of NH4OH for simultaneous detoxification and addition of nitrogen source. Treatment with NH4OH compared favorably with Ca(OH)2, Mg(OH)2, Ba(OH)2, and NaOH to improve fermentability using Saccharomyces cerevisiae. Analysis of monosaccharides, furan aldehydes, phenols, and aliphatic acids was performed after the different treatments. The NH4OH treatments, performed at pH 10.0, resulted in a substantial decrease in the concentrations of furfural and hydroxymethylfurfural. Under the conditions studied, NH4OH treatments gave better results than Ca(OH)2 treatments. The addition of an extra nitrogen source in the form of NH4Cl at pH 5.5 did not result in any improvement in fermentability that was comparable to NH4OH treatments at alkaline conditions. The addition of CaCl2 or NH4Cl at pH 5.5 after treatment with NH4OH or Ca(OH)2 resulted in poorer fermentability, and the negative effects were attributed to salt stress. The results strongly suggest that the highly positive effects of NH4OH treatments are owing to chemical conversions rather than stimulation of the yeast cells by ammonium ions during the fermentation.

  • 2.
    Alriksson, Björn
    et al.
    Karlstad University, Faculty of Technology and Science.
    Sjöde, Anders
    Karlstad University, Faculty of Technology and Science.
    Nilvebrant, Nils-Olof
    STFI-Packforsk, Stockholm, Sweden.
    Jönsson, Leif J.
    Karlstad University, Faculty of Technology and Science.
    Optimal conditions for alkaline detoxification of dilute-acid lignocellulose hydrolysates.2006In: Applied Biochemistry and Biotechnology, ISSN 0273-2289, E-ISSN 1559-0291, Vol. 129-132, p. 599-611Article in journal (Refereed)
    Abstract [en]

    Alkaline detoxification strongly improves the fermentability of dilute-acid hydrolysates in the production of bioethanol from lignocellulose with Saccharomyces cerevisiae. New experiments were performed with NH4OH and NaOH to define optimal conditions for detoxification and make a comparison with Ca(OH)2 treatment feasible. As too harsh conditions lead to sugar degradation, the detoxification treatments were evaluated through the balanced ethanol yield, which takes both the ethanol production and the loss of fermentable sugars into account. The optimization treatments were performed as factorial experiments with 3-h duration and varying pH and temperature. Optimal conditions were found roughly in an area around pH 9.0/60 degrees C for NH4OH treatment and in a narrow area stretching from pH 9.0/80 degrees C to pH 12.0/30 degrees C for NaOH treatment. By optimizing treatment with NH4OH, NaOH, and Ca(OH)2, it was possible to find conditions that resulted in a fermentability that was equal or better than that of a reference fermentation of a synthetic sugar solution without inhibitors, regardless of the type of alkali used. The considerable difference in the amount of precipitate generated after treatment with different types of alkali appears critical for industrial implementation.

  • 3.
    Bohlin, Christina
    et al.
    Karlstad University, Faculty of Technology and Science.
    Jönsson, Leif J.
    Karlstad University, Faculty of Technology and Science.
    Roth, Robyn
    van Zyl, Willem H.
    Department of Microbiology, University of Stellenbosch, South Africa.
    Heterologous Expression of Trametes versicolor Laccase in Pichia pastoris and Aspergillus niger2006In: Applied Biochemistry and Biotechnology, ISSN 0273-2289, E-ISSN 1559-0291, Vol. 129, no 1-3, p. 195-214Article in journal (Refereed)
  • 4.
    Cassland, Pierre
    et al.
    Applied Microbiology, Lund University.
    Sjöde, Anders
    Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
    Winestrand, Sandra
    Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
    Jönsson, Leif J.
    Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
    Nilvebrant, Nils-Olof
    Karlstad University, Faculty of Technology and Science, Department of Chemical Engineering.
    Evaluation of Oxalate Decarboxylase and Oxalate Oxidase for Industrial Applications2010In: Applied Biochemistry and Biotechnology, ISSN 0273-2289, E-ISSN 1559-0291, Vol. 161, p. 255-263Article in journal (Refereed)
  • 5.
    Horváth, Ilona Sárvári
    et al.
    Karlstad University, Division for Chemistry.
    Sjöde, Anders
    Karlstad University, Division for Chemistry.
    Alriksson, Björn
    Karlstad University, Division for Chemistry.
    Jönsson, Leif J
    Karlstad University, Division for Chemistry.
    Nilvebrant, Nils-Olof
    Karlstad University, Division for Chemistry.
    Critical conditions for improved fermentability during overliming of acid hydrolysates from spruce.2005In: Applied Biochemistry and Biotechnology, ISSN 0273-2289, E-ISSN 1559-0291, Vol. 121-124, p. 1031-44Article in journal (Refereed)
    Abstract [en]

    Bioethanol can be produced from wood via acid hydrolysis, but detoxification is needed to achieve good fermentability. Overliming was investigated in a factorial designed experiment, in which pH and temperature were varied. Degradation of inhibitory furan aldehydes was more extensive compared to monosaccharides. Too harsh conditions led to massive degradation of sugars and formation of inhibiting acids and phenols. The ethanol productivity and yield after optimal overliming reached levels exceeding reference fermentations of pure glucose. A novel metric, the balanced ethanol yield, which takes both ethanol production and losses of fermentable sugars into account, was introduced and showed the optimal conditions within the investigated range. The findings allow process technical and economical considerations to govern the choice of conditions for overliming.

  • 6.
    Martin, Carlos
    et al.
    Karlstad University, Faculty of Technology and Science.
    Alriksson, Björn
    Karlstad University, Faculty of Technology and Science.
    Sjöde, Anders
    Karlstad University, Faculty of Technology and Science.
    Nilvebrant, Nils-Olof
    STFI-Packforsk, Stockholm.
    Jönsson, Leif J.
    Karlstad University, Faculty of Technology and Science.
    Dilute sulfuric acid pretreatment of agricultural and agro-industrial residues for ethanol production2007In: Applied Biochemistry and Biotechnology, ISSN 0273-2289, E-ISSN 1559-0291, Vol. 136-140, p. 339-352Article in journal (Refereed)
  • 7.
    Sjöde, Anders
    et al.
    Karlstad University, Faculty of Technology and Science.
    Alriksson, Björn
    Karlstad University, Faculty of Technology and Science.
    Jönsson, Leif J.
    Karlstad University, Faculty of Technology and Science.
    Nilvebrant, Nils-Olof
    STFI-Packforsk, Stockholm, Sweden.
    The potential in bioethanol production from waste fiber sludges in pulp mill-based biorefineries.2007In: Applied Biochemistry and Biotechnology, ISSN 0273-2289, E-ISSN 1559-0291, Vol. 136-140, p. 327-338Article in journal (Refereed)
1 - 7 of 7
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