Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Methacrylate monolithic stationary phases for gradient elution separations in microfluidic devices
Univ Amsterdam, Analyt Chem Grp, Vant Hoff Inst Mol Sci, NL-1090 GD Amsterdam, Netherlands..
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.
Univ Amsterdam, Analyt Chem Grp, Vant Hoff Inst Mol Sci, NL-1090 GD Amsterdam, Netherlands..ORCID iD: 0000-0002-9167-7716
Univ Amsterdam, Analyt Chem Grp, Vant Hoff Inst Mol Sci, NL-1090 GD Amsterdam, Netherlands..
2011 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1218, no 31, p. 5292-5297Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

Methacrylate monolithic stationary phases were produced in fused-silica chips by UV initiation. Poly(butyl methacrylate-co-ethylene dimethacrylate) (BMA) and poly(lauryl methacrylate-co-ethylene dimethacrylate) (LMA) monoliths containing 30, 35 and 40% monomers were evaluated for the separation of peptides under gradient conditions. The peak capacity was used as an objective tool for the evaluation of the separation performance. LMA monoliths of the highest density gave the highest peak capacities (approximate to 40) in gradients of 15 min and all LMA monoliths gave higher peak capacities than the BMA monoliths with the same percentage of monomers. Increasing the gradient duration to 30 min did not increase the peak capacity significantly. However, running fast (5 min) gradients provides moderate peak capacities (approximate to 20) in a short time. Due to the system dead volume of 1 mu L and the low bed volume of the chip, early eluting peptides migrated over a significant part of the column during the dwell time under isocratic conditions. It was shown that this could explain an increased band broadening on the monolithic stationary phase materials used. The effect is stronger with BMA monoliths, which partly explains the inferior performance of this material with respect to peak capacity. The configuration of the connections on the chip appeared to be critical when fast analyses were performed at pressures above 20 bar. (C) 2011 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
2011. Vol. 1218, no 31, p. 5292-5297
Keyword [en]
Methacrylate monoliths, Peptide separations, Gradient elution, Microfluidic devices
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-40611DOI: 10.1016/j.chroma.2011.06.046ISI: 000293432200033PubMedID: 21733523OAI: oai:DiVA.org:kau-40611DiVA, id: diva2:905476
Available from: 2016-02-22 Created: 2016-02-22 Last updated: 2017-11-30Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Schoenmakers, Peter J.

Search in DiVA

By author/editor
Schoenmakers, Peter J.
By organisation
Department of Engineering and Chemical Sciences
In the same journal
Journal of Chromatography A
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 16 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf