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Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate
Univ Helsinki, Dept Chem, Analyt Chem Lab, FIN-00014 Helsinki, Finland..
Univ Helsinki, Dept Chem, Analyt Chem Lab, FIN-00014 Helsinki, Finland.;Natl Univ San Luis, CONICET, Dept Chem, INQUISAL,Lab Analyt Chem, San Luis, Argentina..
Univ Helsinki, Dept Chem, Analyt Chem Lab, FIN-00014 Helsinki, Finland..
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
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2011 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 83, no 15, 6040-6046 p.Article in journal (Refereed) PublishedText
Abstract [en]

Two different experimental approaches were used for obtaining a comprehensive view and understanding of the interactions between apolipoprotein B-100 (ApoB-100) of low-density lipoprotein and apolipoprotein E (ApoE) of high-density lipoprotein and chondroitin-6-sulfate (C6S) of arterial proteoglycan. The techniques employed were partial filling affinity capillary electrophoresis (PF-ACE) and continuous flow quartz crystal inicrobalance (QCM). In addition, molecular dynamic (MD) simulations were used to provide a supportive visual insight into the interaction mechanism. A new tool for analysis of QCM-data was utilized, i.e., adsorption energy distribution calculations, which allowed a deeper understanding of the interactions, especially at different temperatures. The PF-ACE technique probed mainly the strong adsorption interactions whereas in the MD calculations short:- and long-range interactions could be distinguished. Although there are differences in the techniques, a pretty good agreement was achieved between the three approaches for the interaction of 19 amino acid peptide of ApoB with C6S giving log affinity constants of 4.66 by QCM, 5.02 by PP-ACE, and 7.39 by MD, and for 15 amino acid peptide of ApoE with C6S 5.34 by QCM, 5.28 by PT-ACE, and 4.60 by MD at physiological temperature 37.0 degrees C.

Place, publisher, year, edition, pages
2011. Vol. 83, no 15, 6040-6046 p.
National Category
Analytical Chemistry
Research subject
Chemistry
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URN: urn:nbn:se:kau:diva-40612DOI: 10.1021/ac201110cISI: 000293252500034PubMedID: 21651232OAI: oai:DiVA.org:kau-40612DiVA: diva2:905475
Available from: 2016-02-22 Created: 2016-02-22 Last updated: 2016-02-26Bibliographically approved

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Samuelsson, JorgenFornstedt, Torgny
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