Change search
ReferencesLink to record
Permanent link

Direct link
On-Line Whole Blood Analysis Using Microextraction by Packed Sorbent and LC-MS-MS
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences. AstraZeneca R&D Sodertalje, Global DMPK, Sodertalje, Sweden.;Karlstad Univ, Fac Sci & Technol, Dept Chem & Biomed Sci, Karlstad, Sweden..ORCID iD: 0000-0002-3862-8878
2011 (English)In: LC GC North America, ISSN 1527-5949, E-ISSN 1939-1889, Vol. 29, no 7, 612-618 p.Article in journal (Refereed) PublishedText
Abstract [en]

Microextraction by packed sorbent (MEPS) is a new technique for sample preparation that can be connected on-line with liquid chromatography (LC) or gas chromatography (GC) systems without any modifications. This article describes the use of MEPS in clinical and preclinical studies to quantify different drugs in whole blood samples. MEPS was used to determine cyclophosphamide in mouse blood from preclinical g studies using 20 mu L of blood samples. The interday accuracies and 0 precisions ranged from 107-109% and from 2.0-7.0%, respectively. The determination of four immunosuppressive drugs in human blood by MEPS and liquid chromatography-mass spectrometry (LC-MS) is described. The method showed a good selectivity and sensitivity. The calibration curves for everolimus, sirolimus, and tacrolimus ranged from 0.5 to 50 ng/mL and for cyclosporine from 3.0 to 1500 ng/mL. Intraday precisions for the studied immunosuppressive drugs were 2.0-11.7% and interday precision ranged from 5.1 to 13.7% (CV).

Place, publisher, year, edition, pages
2011. Vol. 29, no 7, 612-618 p.
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Biomedical Sciences
Identifiers
URN: urn:nbn:se:kau:diva-40615ISI: 000293320900008OAI: oai:DiVA.org:kau-40615DiVA: diva2:905472
Available from: 2016-02-22 Created: 2016-02-22 Last updated: 2016-02-26Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Abdel-Rehim, Mohamed
By organisation
Department of Chemistry and Biomedical Sciences
In the same journal
LC GC North America
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar

Total: 78 hits
ReferencesLink to record
Permanent link

Direct link