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Regulation of genes involved in microbial degradation of chlorate in the chlorate-respiring bacterium Ideonella dechloratans.
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.
2013 (English)Conference paper, Poster (Other academic)
Abstract [en]

Regulation of genes involved in microbial degradation of chlorate in the chlorate-respiring bacterium Ideonella dechloratans

Ideonella dechloratans is a facultative anaerobe and can use both oxygen and chlorate as terminal electron acceptors. Chlorate metabolism involves two enzymes; chlorate reductase (Clr) and chlorite dismutase (Cld). The genes are located in a cluster for chlorate metabolism that also includes the genes for a cytochrome c and a mob B gene. A possible function of the cyt c gene is electron transport during chlorate reduction but so far no corresponding protein has been found. Our aim is to study the expression and possible regulation of Cld and Clr during aerobic and anaerobic metabolism and to examine if the cytochrome c is expressed in I. dechloratans. We have previously reported expression of Clr and Cld, measured both at the mRNA level and as enzyme activities (1). In order to examine sequences important for gene regulation the upstream regions of Clr and Cld were cloned and inserted in a reporter vector and transformed into E. coli XL-1 Blue. The expression of the cyt c gene was analyzed with qRT-PCR in RNA preparations from cells grown under different growth conditions; aerobically or anaerobically. Our results show that the cloned upstream regions of Clr and Cld include functional promoter sequences and that cyt c is expressed in I. dechloratans with increased levels at growth without an external oxygen supply.

1.            Lindqvist, M. H., N. Johansson, T. Nilsson, and M. Rova. 2012. Expression of Chlorite Dismutase and Chlorate Reductase in the Presence of Oxygen and/or Chlorate as the Terminal Electron Acceptor in Ideonella dechloratans. Appl. Environ. Microbiol. 78:4380-4385.

 

Place, publisher, year, edition, pages
2013. 312-312 p.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:kau:diva-33948OAI: oai:DiVA.org:kau-33948DiVA: diva2:752174
Conference
5th Congress of European Microbiologists (FEMS 2013), Leipzig, Germany, July 21-25, 2013.
Available from: 2014-10-02 Created: 2014-10-02 Last updated: 2015-07-07Bibliographically approved

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Hellberg Lindqvist, MiriamNilsson, ThomasRova, Maria
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