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Ellagic acid inhibits lipopolysaccharide-induced expression of enzymesinvolved in the synthesis of prostaglandin E2 in human monocytes
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Health Sciences.
2010 (English)In: British Journal of Nutrition, ISSN 0007-1145, E-ISSN 1475-2662, Vol. 103, 1102-1109 p.Article in journal (Refereed) Published
Abstract [en]

Ellagic acid, a natural polyphenol found in certain fruits, nuts and vegetables, has in recent years been the subject of intense research within the fields of cancer and inflammation. Pain, fever and swelling, all typical symptoms of inflammation, are ascribed to elevated levels of PGE(2). In the present study, we have investigated the effects of ellagic acid on PGE(2) release and on prostaglandin-synthesising enzymes in human monocytes. Ellagic acid was found to inhibit Ca ionophore A23187-, phorbol myristate acetate- and opsonised zymosan-induced release of PGE(2) from monocytes pre-treated with the inflammatory agent lipopolysaccharide. Ellagic acid suppressed the lipopolysaccharide-induced increase in protein expression of cyclo-oxygenase-2 (COX-2), microsomal PGE synthase-1 (mPGEs-1) and cytosolic phospholipase A(2)alpha (cPLA(2)alpha), while it had no effect on the constitutively expressed COX-1 protein. Ellagic acid had no apparent inhibitory effect on these enzymes when the activities were determined in cell-free assays. We conclude that the inhibitory effect of ellagic acid on PGE(2) release from monocytes is due to a suppressed expression of COX-2, mPGEs-1 and cPLA(2)alpha, rather than a direct effect on the activities of these enzymes.

Place, publisher, year, edition, pages
Cambridge University Press, 2010. Vol. 103, 1102-1109 p.
Keyword [en]
CYTOSOLIC PHOSPHOLIPASE A(2); POMEGRANATE JUICE CONSUMPTION; FACTOR-KAPPA-B; ARACHIDONIC-ACID; CANCER-CELLS; E SYNTHASE-1; SIGNALING PATHWAYS; ENDOTHELIAL-CELLS; IN-VITRO; MACROPHAGES
National Category
Basic Medicine
Research subject
Biology
Identifiers
URN: urn:nbn:se:kau:diva-30104DOI: 10.1017/S0007114509992935ISI: 000277248800004PubMedID: 19948080OAI: oai:DiVA.org:kau-30104DiVA: diva2:666104
Funder
County Council of Östergötland
Available from: 2013-11-21 Created: 2013-11-21 Last updated: 2014-10-23Bibliographically approved

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Karlsson, SofiaNånberg, EewaFjaeraa Alfredsson, ChristinaWijkander, Jonny
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Department of Chemistry and Biomedical SciencesDepartment of Health Sciences
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