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Platelet-derived growth factor increases the turnover of GTP/GDP on ras in permeabilized fibroblasts.
Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för genetik och patologi.
1993 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 268, no 24, p. 18187-18194Article in journal (Refereed) Published
Abstract [en]

The potent mitogen platelet-derived growth factor (PDGF) induced a rapid increase in Ras.GTP in permeabilized human and murine fibroblasts. The effect was initiated by both PDGF-AA acting exclusively through PDGF alpha-receptors, and by PDGF-BB interacting with both alpha- and beta-type receptors. The dose-response curves suggest that both receptor types mediate the response. PDGF-dependent Ras activation, measured as increased formation of Ras.GTP, was rapid and reversible. At 37 degrees C the effect had a duration of around 10 min. The PDGF-dependent increase in Ras.GTP was followed by a simultaneous increase in Ras.GDP. Under no experimental condition could a relative increase in Ras.GTP be detected. 0.5 microM GDP and 0.5 microM GTP were equally potent competing for the formation of Ras.[alpha-32P]GTP upon PDGF stimulation. Furthermore, when the basal nucleotide exchange rate on Ras was elevated by omission of Mg2+ from the medium, PDGF had no further effect on the formation of Ras.GTP. We therefore conclude that PDGF activates Ras through a mechanism leading to an increased nucleotide exchange on Ras.

Place, publisher, year, edition, pages
1993. Vol. 268, no 24, p. 18187-18194
National Category
Medical and Health Sciences
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URN: urn:nbn:se:kau:diva-29812PubMedID: 8349694OAI: oai:DiVA.org:kau-29812DiVA, id: diva2:657607
Available from: 2013-10-21 Created: 2013-10-20 Last updated: 2017-12-06Bibliographically approved

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