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Protein kinase C remains functionally active during TPA induced neuronal differentiation of SH-SY5Y human neuroblastoma cells
Department of Pathology, University Hospital, Uppsala.
Department of Biochemistry and Pharmacy, Åbo Akademi University, Finland.
Department of Biochemistry and Pharmacy, Åbo Akademi University, Finland.
Department of Pathology, University Hospital, Uppsala.
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1992 (English)In: Journal of Cellular Physiology, ISSN 0021-9541, E-ISSN 1097-4652, Vol. 152, no 3, 536-44 p.Article in journal (Refereed) Published
Abstract [en]

SH-SY5Y human neuroblastoma cells can be induced to differentiate into a neuronal phenotype by treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). In other cell systems, TPA treatment frequently leads to down-regulation of protein kinase C (PKC). However, we now report that TPA-treated and non-treated SH-SY5Y cells express PKC-alpha, but not PKC-beta and PKC-gamma, mRNA. Furthermore, only a slight down-regulation of the PKC-alpha protein could be seen during prolonged treatment with 16 nM TPA, the concentration giving optimal differentiation. In contrast, a higher concentration of TPA (1.6 microM) results in a poor neuronal differentiation and a complete down-regulation of PKC-alpha. PKC-alpha was rapidly translocated to the particulate fraction and remained membrane bound for at least 4 days during treatment with 16 nM TPA. In such cells a sustained increased level of the phosphorylated form of a 80,000 Dalton PKC-substrate was found. In addition to this sustained augmented phosphorylation, administration of fresh TPA at day 4 caused a small but reproducible further increased level of phosphorylated substrate. When the PKC activity was measured by the histone phosphorylation assay a substantial fraction of the initial enzyme activity could still be detected after 4 days of TPA treatment. Taken together, the data demonstrate that PKC remains functionally active during TPA induced differentiation of SH-SY5Y cells, which may suggest a continuous role for the enzyme during the differentiation process.

Place, publisher, year, edition, pages
1992. Vol. 152, no 3, 536-44 p.
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Medical and Health Sciences
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URN: urn:nbn:se:kau:diva-29814DOI: 10.1002/jcp.1041520313PubMedID: 1506412OAI: oai:DiVA.org:kau-29814DiVA: diva2:657605
Available from: 2013-10-21 Created: 2013-10-20 Last updated: 2013-10-29Bibliographically approved

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