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Temporal relationship between inositol polyphosphate formation and increases in cytosolic Ca2+ in quiescent 3T3 cells stimulated by platelet-derived growth factor, bombesin and vasopressin.
Imperial Cancer Research Fund, London, UK.
1988 (English)In: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 7, no 9, 2741-7 p.Article in journal (Refereed) Published
Abstract [en]

We determined the temporal relationship between the formation of inositol phosphates and increase in cytosolic [Ca2+] elicited by bombesin, vasopressin and platelet-derived growth factor (PDGF) in quiescent Swiss 3T3 cells. These responses were measured under identical conditions. Bombesin caused a rapid increase in inositol 1,4,5-trisphosphate which coincided with the increase in cytosolic [Ca2+]. This was followed by a slower but marked increase in inositol 1,3,4-trisphosphate and inositol-bisphosphate. Vasopressin elicited a similar sequence of events. In sharp contrast, highly purified porcine PDGF induced increases in cytosolic [Ca2+] and inositol 1,4,5-trisphosphate that were temporally uncoupled: detectable inositol polyphosphate formation occurred after Ca2+ mobilization from intracellular stores. The same temporal dissociation was observed when a recombinant v-sis product was used instead of porcine PDGF. However, PDGF was as effective as bombesin in stimulating the formation of inositol phosphates after 5-10 min of incubation. The data suggest that PDGF increases cytosolic [Ca2+] via a different signal transduction pathway from that utilized by bombesin and vasopressin. These findings have important implications for understanding the signal transduction pathway activated by PDGF.

Place, publisher, year, edition, pages
1988. Vol. 7, no 9, 2741-7 p.
National Category
Medical and Health Sciences
Research subject
Biomedical Sciences
URN: urn:nbn:se:kau:diva-29817PubMedID: 3181139OAI: diva2:657601
Available from: 2013-10-21 Created: 2013-10-20 Last updated: 2013-10-28Bibliographically approved

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