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Basic FGF and IGF-I promote differentiation of human SH-SY5Y neuroblastoma cells in culture.
Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för genetik och patologi.
1994 (English)In: Growth Factors, ISSN 0897-7194, E-ISSN 1029-2292, Vol. 10, no 1, p. 29-39Article in journal (Refereed) Published
Abstract [en]

Phorbolester-triggered differentiation of SH-SY5Y neuroblastoma cells requires serum and a prolonged activation of protein kinase C (PKC). Under serum-free conditions development of a mature phenotype requires phorbolester in combination with a member of either the insulin-like growth factor (IGF) or the platelet-derived growth factor family. Here we report that basic and acidic fibroblast growth factor (FGF) and epidermal growth factor, but not nerve growth factor, synergistically potentiate phorbolester-induced differentiation. Alone these factors induced a mitogenic response which varied in magnitude, with basic FGF and IGF-I being the two most potent mitogens. However, a combination of basic FGF and IGF-I induced differentiation as judged by morphology and the increase in growth associated protein (GAP-43) and neuropeptide tyrosine mRNA levels. In contrast to the phenotype obtained in the presence of phorbolester, bFGF and IGF-I-treated SH-SY5Y cells retained their capacity to proliferate. Finally, in these cells, the phosphorylation of the endogenous PKC substrate, myristoylated alanine-rich C-kinase substrate (MARCKS), was slightly increased during several days, suggesting an involvement of PKC in the bFGF and IGF-I-induced differentiation.

Place, publisher, year, edition, pages
1994. Vol. 10, no 1, p. 29-39
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:kau:diva-29811PubMedID: 7514011OAI: oai:DiVA.org:kau-29811DiVA, id: diva2:657579
Available from: 2013-10-20 Created: 2013-10-20 Last updated: 2017-12-06Bibliographically approved

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