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Relative importance of column and adsorption parameters on the productivity in preparative liquid chromatography. I: Investigation of a chiral separation system
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.ORCID iD: 0000-0003-1819-1709
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
2013 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1299, 58-63 p.Article in journal (Refereed) Published
Abstract [en]

Starting out from an experimental chiral separation system we have used computer simulations for a systematic investigation on how the maximum productivity depends on changes in column length, packing particle size, column efficiency, back pressure, sample concentration/solubility, selectivity, retention factor of the first eluting component and monolayer saturation capacity. The study was performed by changing these parameters, one at a time, and then calculating the corresponding change in maximum productivity. The three most important parameters for maximum production rate was found to be (i) the selectivity (ii) the retention factor of the first eluting component and (iii) the column length. Surprisingly, the column efficiency and sample concentration/solubility were of minor importance. These findings can be used as rough guidelines for column selection, e.g. a low-efficiency column are more likely perform better, in terms of productivity, than a high-efficiency column that have higher retention factor for the first eluting component.

Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 1299, 58-63 p.
Keyword [en]
Preparative chromatography, Process optimization, Productivity
National Category
Analytical Chemistry Chemical Engineering
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-28801DOI: 10.1016/j.chroma.2013.05.031ISI: 000321424000008PubMedID: 23769205OAI: oai:DiVA.org:kau-28801DiVA: diva2:643989
Available from: 2013-08-29 Created: 2013-08-29 Last updated: 2016-10-06Bibliographically approved

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Forssén, PatrikSamuelsson, JörgenFornstedt, Torgny
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