Evaluation of a combined linear–nonlinear approach for column characterization using modern alkaline-stable columns as model
2013 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 36, no 11, 1753-1761 p.Article in journal (Refereed) Published
This study investigates if deeper understanding is achieved when combining nonlinear and linear chromatographic column characterization methods. As test systems, two hybrid columns (Phenomenex Gemini-NX C18 and Kromasil Eternity C18) and one classic one (Kromasil-C18) were selected. The nonlinear methods were based on firm adsorption theory and involved determination of adsorption isotherms followed by calculations with a new numerical tool, adsorption energy distribution, on probe components at different pH values. The linear methods involved the hydrophobic subtraction model and selected probe components retention factors as a function of pH. The combined analysis indicated that both complementary and confirmative information can be achieved regarding the actual model systems.
Place, publisher, year, edition, pages
2013. Vol. 36, no 11, 1753-1761 p.
Adsorption isotherms, Characterization, Linear methods, Nonlinear methods, Retention
Research subject Chemistry
IdentifiersURN: urn:nbn:se:kau:diva-28105DOI: 10.1002/jssc.201201132ISI: 000319919900009PubMedID: 23554373OAI: oai:DiVA.org:kau-28105DiVA: diva2:631182