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On the Reproducibility between Different Modern Supercritcal Fluid Chromatographic Systems
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.ORCID iD: 0000-0003-1819-1709
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.
2013 (English)Conference paper (Other academic)
Abstract [en]

Three different, commercially available, Supercritical Fluid Chromatography (SFC) systems were investigated: Thar Super Pure Discovery Series SFC, Waters UPC2 and Agilent 1260 Infinity SFC. The reason for choosing the two analytical systems from Agilent and Waters is that they represent two of the latest commercial systems available while the semi-preparative instrument was added to widen the study to include instruments also used for preparative purposes. With identical operational conditions set these systems were used to acquire analytical retention data and adsorption isotherms from overloaded injections. The investigation was limited to the use of methanol as modifier and operational conditions, temperature and back pressure most typically observed when utilizing SFC to separate polar compounds. The results clearly show that both analytical retention times and elution profiles are system dependent. Since the overloaded elution profiles are system dependent the adsorption isotherm will also be different. However, this do not mean that the adsorption is different, instead this it is due to the fact that identical instrumental settings, especially pressure and modifier composition settings, does not necessarily mean that the conditions inside the column are identical. This means that it is not possible to transfer an established separation method from one system to another, even if one is using the same column and identical instrument settings. Understanding of SFC-systems will be of fundamental importance for successful transfer of methods between systems, reliable adsorption isotherm determination, and analytical quality work and scaling up from analytical to preparative mode. These issues can probably be solved by measuring mass flow, pressure and temperature along the column, together with a sound understanding of the density variations of the mobile phase. However, the work of finding acceptable applications or guidelines to remove the tedious need for these measurements is currently investigated in our lab. This is a contribution from the Fundamental Separation Science Group www.FSSG.se

Place, publisher, year, edition, pages
Amsterdam, 2013.
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-28108OAI: oai:DiVA.org:kau-28108DiVA: diva2:631181
Conference
39th International Symposium on High Performance Liquid Phase Separations and Related Techniques, AMSTERDAM: June 16 - 20, 2013
Available from: 2013-06-20 Created: 2013-06-20 Last updated: 2014-12-12Bibliographically approved

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Enmark, MartinSamuelsson, JörgenFornstedt, Torgny
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