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Use of Dimethylacrylamide Coated Capillaries for the Study of beta2-Glycoprotein I with Affinity Capillary Electrophoresis
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
2003 (English)In: / [ed] Lars G. Blomberg, Niels H. H. Heegaard, 2003Conference paper (Other (popular science, discussion, etc.))
Abstract [en]

The purpose of this work is to develop a solution method for examining interactions of human beta2-Glycoprotein I (b2gpI) with antibodies and natural ligands under nondenaturing conditions. In the approach, a physiological pH was applied because our intention was to study the interactions between b2gpI and antibodies and the protein must thus be kept in its native state. Preliminary experiments have shown that the recovery of the protein, when analyzed in an uncoated capillary, is poor. At physiological pH the protein is positively charged and most likely it was adsorbed at the negatively charged capillary wall in these experiments. Therefore dimethylacrylamide (DMA) coated capillaries1 are used and analyses could be performed with acceptable recovery. Interaction studies are performed simply by adding the antibody or ligand to the running buffer. The method showed good repeatability and efficiency

Place, publisher, year, edition, pages
2003.
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-25266OAI: oai:DiVA.org:kau-25266DiVA: diva2:599042
Conference
Analysdagarna 2003, Göteborg
Available from: 2013-01-22 Created: 2013-01-22 Last updated: 2013-01-22

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