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Application of Plasma Desorption Mass Spectrometry to Molecular Weight Determination of Structural Protein from Insect Cuticle
Department of Molecular Biology, Odense University, Denmark.
Department of Molecular Biology, Odense University, Denmark.
Tandem Accelerator Laboratory, Uppsala University.
Tandem Accelerator Laboratory, Uppsala University.
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1986 (English)In: Journal of Mass Spectrometry, ISSN 1076-5174, E-ISSN 1096-9888, Vol. 13, no 12, 689-691 p.Article in journal (Refereed) Published
Abstract [en]

During sequence determination of a structural protein from the cuticle of the migratory locust, Locusta migratoria, a discrepancy was found between the molecular weight calculated from the sequence data (15 323) and that estimated by SDS-gel electrophoresis (21 600). The protein contains several repeated sequences, and the discrepancy might indicate that part of the sequence was missing or that the protein contained a large prosthetic group. The molecular weight of the protein was determined by 127I-plasma desorption mass spectrometry to be 15 329±50 which confirms the sequence data and shows the molecular weight determination based on SDS-electrophoresis to be too high.

Place, publisher, year, edition, pages
1986. Vol. 13, no 12, 689-691 p.
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Biochemistry and Molecular Biology
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URN: urn:nbn:se:kau:diva-16970DOI: 10.1002/bms.1200131209OAI: oai:DiVA.org:kau-16970DiVA: diva2:590571
Available from: 2013-01-21 Created: 2013-01-21 Last updated: 2014-09-30Bibliographically approved

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