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New Techniques for Sample Preparation in Analytical Chemistry: Microextraction in Packed Syringe (MEPS) and Methacrylate Based Monolithic Pipette Tips
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
2008 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

Sample preparation is often a bottleneck in systems for chemical analysis. The aim of this work was to investigate and develop new techniques to address some of the shortcomings of current sample preparation methods. The goal has been to provide full automation, on-line coupling to detection systems, short sample preparation times and high-throughput.

In this work a new technique for sample preparation that can be connected on-line to liquid chromatography (LC) and gas chromatography (GC) has been developed. Microextraction in packed syringe (MEPS) is a new solid-phase extraction (SPE) technique that is miniaturized and can be fully automated. In MEPS approximately 1 mg of sorbent material is inserted into a gas tight syringe (100-250 μL) as a plug. Sample preparation takes place on the packed bed. Evaluation of the technique was done by the determination of local anaesthetics in human plasma samples using MEPS on-line with LC and tandem mass spectrometry (MS-MS). MEPS connected to an autosampler was fully automated and clean-up of the samples took about one minute. In addition, in the case of plasma samples the same plug of sorbent could be used for about 100 extractions before it was discarded.

A further aim of this work was to increase sample preparation throughput. To do that disposable pipette tips were packed with a plug of porous polymer monoliths as sample adsorbent and were then used in connection with 96-well plates and LC-MS-MS. The evaluation of the methods was done by the analysis of local anaesthetics lidocaine and ropivacaine, and anti-cancer drug roscovitine in plasma samples. When roscovitine and lidocaine in human plasma and water samples were used as model substances, a 96-plate was handled in about two minutes. Further, disposable pipette tips may be produced at low cost and because they are used only once, carry-over is eliminated.

Place, publisher, year, edition, pages
Fakulteten för teknik- och naturvetenskap , 2008. , p. 66
Series
Karlstad University Studies, ISSN 1403-8099 ; 2008:4
Keywords [en]
Liquid Chromatography/Tandem Mass Spectrometry, Local Anaesthetics, Microextraction in Packed Syringe, Monoliths, Pipette Tips, Sample Preparation
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-1420ISBN: 978-91-7063-161-0 (print)OAI: oai:DiVA.org:kau-1420DiVA, id: diva2:5312
Public defence
2008-02-22, 9C204, Universitetsgatan 2, 651 88, Karlstad, 10:15
Opponent
Supervisors
Available from: 2008-02-12 Created: 2008-02-12 Last updated: 2011-11-09
List of papers
1. Microextraction in packed syringe (MEPS) for liquid and gas chromatographic applications: Part II - Determination of ropivacaine and its metabolites in human plasma samples using MEPS with liquid chromatography/tandem mass spectrometry
Open this publication in new window or tab >>Microextraction in packed syringe (MEPS) for liquid and gas chromatographic applications: Part II - Determination of ropivacaine and its metabolites in human plasma samples using MEPS with liquid chromatography/tandem mass spectrometry
2004 (English)In: Journal of Mass Spectrometry, ISSN 1076-5174, E-ISSN 1096-9888, Vol. 39, no 12, p. 1488-1493Article in journal (Refereed) Published
Identifiers
urn:nbn:se:kau:diva-1991 (URN)10.1002/jms.731 (DOI)
Available from: 2008-02-12 Created: 2008-02-12 Last updated: 2017-12-07Bibliographically approved
2. New trends in sample preparation: on-line microextraction in packed syringe (MEPS) for LC and GC applications Part III: Determination and validation of local anaesthetics in human plasma samples using a cation-exchange sorbent, and MEPS–LC–MS–MS
Open this publication in new window or tab >>New trends in sample preparation: on-line microextraction in packed syringe (MEPS) for LC and GC applications Part III: Determination and validation of local anaesthetics in human plasma samples using a cation-exchange sorbent, and MEPS–LC–MS–MS
2004 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 813, no 1-2, p. 129-135Article in journal (Refereed) Published
Identifiers
urn:nbn:se:kau:diva-1972 (URN)10.1016/j.jchromb.2004.09.020 (DOI)
Available from: 2008-02-05 Created: 2008-02-05 Last updated: 2017-12-07Bibliographically approved
3. Increasing Sample Preparation Throughput Using Monolithic Methacrylate Polymer as Packing Material for 96-Tip Robotic Device
Open this publication in new window or tab >>Increasing Sample Preparation Throughput Using Monolithic Methacrylate Polymer as Packing Material for 96-Tip Robotic Device
2006 (English)In: Journal of Liquid Chromatography & Related Technologies, ISSN 1082-6076, E-ISSN 1520-572X, Vol. 29, no 10, p. 1477-1489Article in journal (Refereed) Published
Identifiers
urn:nbn:se:kau:diva-1993 (URN)10.1080/10826070600674919 (DOI)
Available from: 2008-02-12 Created: 2008-02-12 Last updated: 2017-12-07Bibliographically approved
4. Surface modified polypropylene pipette tips packed with a monolithic plug of adsorbent for high-throughput sample preparation
Open this publication in new window or tab >>Surface modified polypropylene pipette tips packed with a monolithic plug of adsorbent for high-throughput sample preparation
2007 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 30, no 12, p. 1964-1972Article in journal (Refereed) Published
Identifiers
urn:nbn:se:kau:diva-1994 (URN)10.1002/jssc.200600523 (DOI)
Available from: 2008-02-12 Created: 2008-02-12 Last updated: 2017-12-07Bibliographically approved
5. Some Factors Affecting the Performance of Microextraction in Packed Syringe (MEPS)
Open this publication in new window or tab >>Some Factors Affecting the Performance of Microextraction in Packed Syringe (MEPS)
(English)Manuscript (Other academic)
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-1995 (URN)
Available from: 2008-02-12 Created: 2008-02-12 Last updated: 2011-11-09Bibliographically approved

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