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New Techniques for Sample Preparation in Analytical Chemistry
Karlstad University, Division for Chemistry.
2005 (English)Licentiate thesis, comprehensive summary (Other scientific)
Abstract [en]

Sample preparation is often a bottleneck in systems for chemical analysis. The aim of this work was to investigate and develop new techniques to address some of the shortcomings of current sample preparation methods. The goal has been to provide full automation, on-line coupling to detection systems, short sample preparation times and high-throughput.

A new technique for sample preparation that can be connected on-line to liquid chromatography (LC) and gas chromatography (GC) has been developed. Microextraction in packed syringe (MEPS) is a new solid-phase extraction (SPE) technique that is miniaturized and can be fully automated. In MEPS approximately 1 mg of sorbent material is inserted into a gas tight syringe (100-250 μL) as a plug. Sample preparation takes place on the packed bed. Evaluation of the technique was done by the determination of local anaesthetics in human plasma samples using MEPS on-line with LC and tandem mass spectrometry (MS-MS). MEPS connected to an autosampler was fully automated and clean-up of the samples took one minute. In addition, in the case of plasma samples the same plug of sorbent could be used for about 100 extractions before it was discarded.

A further aim of this work was to increase sample preparation throughput. To do that disposable pipette tips were packed with a plug of porous polymer monoliths as sample adsorbent and were then used in connection with 96-well plates and LC-MS-MS. When roscovitine in human plasma and water samples was used as model substance, a 96-plate was handled in two minutes.

Place, publisher, year, edition, pages
Institutionen för kemi , 2005.
Series
Karlstad University Studies, ISSN 1403-8099 ; 2005:24
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-1374ISBN: 91-85335-65-7 (print)OAI: oai:DiVA.org:kau-1374DiVA, id: diva2:5262
Presentation
2005-06-15, Ericssonsalen, 9C 204, Karlstads universitet, Karlstad, 10:15
Available from: 2008-02-05 Created: 2008-02-05 Last updated: 2010-09-14
List of papers
1. Microextraction in packed syringe (MEPS) for liquid and gas chromatographic applications: Part II - Determination of ropivacaine and its metabolites in human plasma samples using MEPS with liquid chromatography/tandem mass spectrometry
Open this publication in new window or tab >>Microextraction in packed syringe (MEPS) for liquid and gas chromatographic applications: Part II - Determination of ropivacaine and its metabolites in human plasma samples using MEPS with liquid chromatography/tandem mass spectrometry
2004 (English)In: Journal of Mass Spectrometry, ISSN 1076-5174, E-ISSN 1096-9888, Vol. 39, no 12, p. 1488-1493Article in journal (Refereed) Published
Identifiers
urn:nbn:se:kau:diva-1991 (URN)10.1002/jms.731 (DOI)
Available from: 2008-02-12 Created: 2008-02-12 Last updated: 2017-12-07Bibliographically approved
2. New trends in sample preparation: on-line microextraction in packed syringe (MEPS) for LC and GC applications Part III: Determination and validation of local anaesthetics in human plasma samples using a cation-exchange sorbent, and MEPS–LC–MS–MS
Open this publication in new window or tab >>New trends in sample preparation: on-line microextraction in packed syringe (MEPS) for LC and GC applications Part III: Determination and validation of local anaesthetics in human plasma samples using a cation-exchange sorbent, and MEPS–LC–MS–MS
2004 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 813, no 1-2, p. 129-135Article in journal (Refereed) Published
Identifiers
urn:nbn:se:kau:diva-1972 (URN)10.1016/j.jchromb.2004.09.020 (DOI)
Available from: 2008-02-05 Created: 2008-02-05 Last updated: 2017-12-07Bibliographically approved
3. Increasing Sample Preparation Throughput Using Monolithic Methacrylate Polymer as Packing Material for 96-Tips: 2 Minutes per 96-Well Plate
Open this publication in new window or tab >>Increasing Sample Preparation Throughput Using Monolithic Methacrylate Polymer as Packing Material for 96-Tips: 2 Minutes per 96-Well Plate
2005 (English)Manuscript (preprint) (Other academic)
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kau:diva-1973 (URN)
Available from: 2008-02-05 Created: 2008-02-05 Last updated: 2011-11-09Bibliographically approved

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