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Deformations of overloaded bands under pH-stable conditions in reversed phase chromatography
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.ORCID iD: 0000-0003-1819-1709
2011 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1218, no 15, 1966-1973 p.Article in journal (Refereed)
Abstract

It has recently been demonstrated, using mathematical models, how peculiar overloaded band profiles of basic compounds are due to the local pH in the column when using low capacity buffers. In this study, overloaded peak shapes resulting after injection of carefully pH matched samples close to the pKa of the chosen solute are investigated primarily on two columns; one hybrid silica C18 column (Kromasil Eternity) and one purely polymeric column (PLRP-S), the latter lacking C18 ligands. It was found that distorted peaks of the basic test compound appear even though there is no difference in pH between the injected sample solution and the eluent; the previous explanation to why these effects occur is based on a pH mismatch. Thus, the unusual band shapes are not due to an initial pH difference. Furthermore, it was observed that the effect does not appear on polymeric columns without C18 ligands, but only on columns with C18 ligands, independently of the base matrix (silica, hybrid silica, polymeric)

Place, publisher, year, edition, pages
2011. Vol. 1218, no 15, 1966-1973 p.
Keyword [en]
Hybrid silica column, Polymeric column, Adsorption isotherm, Alkaline conditions, pH stable conditions, Band distortions, Reversed phase, Preparative chromatography, Overloaded bands, Sample pH
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-10530ISI: 000289331200006OAI: oai:DiVA.org:kau-10530DiVA: diva2:494071
Available from: 2012-02-08 Created: 2012-02-08 Last updated: 2017-01-25Bibliographically approved

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http://dx.doi.org/10.1016/j.chroma.2010.09.002

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Fornstedt, TorgnySamuelsson, Jörgen
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