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Injection profiles in liquid chromatography. I. A fundamental investigation
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.ORCID iD: 0000-0003-1819-1709
Department of Physical and Analytical Chemistry, Uppsala University.
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
2010 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1217, no 26, 4306-4312 p.Article in journal (Refereed) Published
Abstract [en]

This is a fundamental experimental and theoretical investigation on how the injection profile depends on important experimental parameters. The experiments revealed that the injection profile becomes more eroded with increased (i) flow rate, (ii) viscosity of the eluent, (iii) size of the solute, (iv) injection volume and (v) inner diameter of the injection loop capillary. These observations cannot be explained by a 1D-convection-diffusion equation, since it does not account for the effect of the parabolic flow and the radial diffusion on the elution profile. Therefore, the 1D model was expanded into a 2D-convection-diffusion equation with cylindrical coordinates, a model that showed a good agreement with the experimental injection profiles dependence on the experimental parameters. For a deeper understanding of the appearance of the injection profile the 2D model is excellent, but to account for injection profiles of various injection volumes and flow rates in preparative and process-chromatography using computer-optimizations, a more pragmatic approach must be developed. The result will give guidelines about how to reduce the extra-column variance caused by the injection profile. This is important both for preparative and analytical chromatography; in particular for modern analytical systems using short and narrow columns

Place, publisher, year, edition, pages
Amsterdam: Elsevier, 2010. Vol. 1217, no 26, 4306-4312 p.
Keyword [en]
Injection profile, Convection-diffusion equation, Finite elements, Injection volume, Flow rate, Computer optimization
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-10528DOI: 10.1016/j.chroma.2010.04.045OAI: oai:DiVA.org:kau-10528DiVA: diva2:494069
Available from: 2012-02-08 Created: 2012-02-08 Last updated: 2017-01-25Bibliographically approved

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Publisher's full texthttp://www.sciencedirect.com/science/article/pii/S0021967310005315

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