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Regulation of the genes for chlorate reductase (Clr) and chlorite dismutase (Cld) in the chlorate-respiring bacterium Ideonella dechloratans
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences. (Skogsindustriell förädling och energiteknik)
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences. (Skogsindustriell förädling och energiteknik)
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences. (Skogsindustriell förädling och energiteknik)
2010 (English)In: FEBS Journal 277(2010) Supplement 1. Poster presentations, Wiley-Blackwell , 2010, B4.62.- p.Conference paper, (Other academic)
Abstract [en]

Enzyme activities and mRNA levels of chlorate reductase and chlorite dismutase was investigated in whole cell extracts of Ideonella dechloratans grown under different growth conditions. This bacterium grows well both at aerobic and anaerobic conditions, using oxygen and chlorate, respectively, as a terminal electron acceptor. It was found that preparations from cells grown in the absence of chlorate under aerobic conditions showed activity of both chlorate reductase, measured as chlorate dependent reduction of methyl viologen, and chlorite dismutase, measured as chlorite dependent oxygen production. At aerobic growth conditions, the addition of chlorate resulted in an increased activity of chlorate reductase. The highest activity of chlorate reductase was found in preparations from cells grown anaerobically in the presence of chlorate. No increase in enzyme activity could be detected for chlorite dismutase during anaerobic or aerobic growth in the presence of chlorate, compared to aerobic growth in the absence of chlorate. The mRNA levels for Clr and Cld, measured by real-time quantitative PCR using 16SrRNA as an intern standard, was found to be equal in preparations from cells grown anaerobically in the presence of chlorate compared to cells grown under aerobic conditions in the absence of chlorate. The results suggest that, in I. dechloratans, the activity of chlorate reductase is up-regulated by at least two factors, anaerobiosis and the presence of chlorate. Interestingly, the results also indicate that the studied regulation occurs at post-transcriptional level, while most examples of oxygen regulation in bacteria are reported to occur at transcriptional level.  

Place, publisher, year, edition, pages
Wiley-Blackwell , 2010. B4.62.- p.
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-10152ISI: 000278565100598OAI: oai:DiVA.org:kau-10152DiVA: diva2:493673
Conference
35th FEBS Congress, Gothenburg, Sweden,June 26-July 1, 2010.
Available from: 2012-02-08 Created: 2012-02-08 Last updated: 2016-02-22Bibliographically approved

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