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Nuclear expression of mu-opioid receptors in a human mesothelial cell line
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
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2009 (English)In: Autonomic & Autacoid Pharmacology, ISSN 1474-8665, E-ISSN 1474-8673, Vol. 29, no 4, 165-170 p.Article in journal (Refereed)
Abstract

1 Possibly acting via mu-opioid receptors (MORs), morphine inhibits the formation of

experimentally induced postoperative abdominal adhesions in rats. Mesothelial cells may

participate in adhesion formation by secreting mediators that interfere negatively with

fibrinolysis. Morphine may prevent adhesions by inhibiting the release of pro-adhesion

mediators from mesothelial cells. This study aimed to investigate whether human mesothelial

cells express MOR-1; if so, such could constitute a site of action for morphine in adhesion

prevention.

2 Cells from Met-5A, a human mesothelial cell line were seeded and prepared for

immunocytochemistry and Western blotting.

3 Immunocytochemistry showed MOR-1 expression in mesothelial cells, predominantly in the

nuclei. Western blotting showed two bands (c. 35 and 50 kDa) which correspond to those

obtained with a control lysate from cells known to express MORs. In addition, we found

MOR-1 expression with nuclear and cytoplasmatic localization in biopsies from human

abdominal adhesions.

4 The current findings may suggest that morphine could interact directly with mesothelial cells

via MOR-1 receptors, and thereby modulate adhesion formation, possibly by interfering with

the release of pro-adhesion factors from these cells

Place, publisher, year, edition, pages
2009. Vol. 29, no 4, 165-170 p.
Keyword [en]
adhesion, G-protein coupled receptor, intracrine, morphine, mu-opioid receptor, nuclear exression
National Category
Biological Sciences
Research subject
Biomedical Science
Identifiers
URN: urn:nbn:se:kau:diva-9982OAI: oai:DiVA.org:kau-9982DiVA: diva2:493496
Available from: 2012-02-08 Created: 2012-02-08 Last updated: 2013-06-12Bibliographically approved

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