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Adenosine A2 receptor presence and synergy with cholinergic stimulation in rabbit lacrimal gland
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
2010 (English)In: Current Eye Research, ISSN 0271-3683, Vol. 35, no 6, 466-474 p.Article in journal (Refereed) Published
Abstract [en]

PURPOSE: Secretion from the lacrimal gland is an important part of the well-being of the eye, and a central part in the search for treatment of dry eye syndrome. Adenosine has stimulatory effects on the lacrimal gland, and can potentiate the effect of the cholinergic agonist carbachol (Cch). The aim of the present study is to investigate the presence of the adenosine A(2) receptor subtypes A(2A) and A(2B) in the rabbit lacrimal gland, and to characterize their role in regulated acinar cell secretion.METHODS: Expression of the receptors was investigated using reverse transcriptase-PCR (RT-PCR) and immunofluorescence, and secretion effects were studied using a secretion assay in isolated lacrimal gland acinar cells.RESULTS: Presence of both receptors was detected by RT-PCR and immunofluorescence. The secretion assay revealed a minor effect of stimulation of the A(2) receptors, and a strong synergistic effect with the cholinergic agonist Cch. The synergistic effect was significantly reduced by the A(2B) antagonist PSB 1115, but not by the A(2A) antagonist SCH 58261, indicating that A(2B) is the receptor responsible for this potentiation.CONCLUSIONS: The study reveals the presence of the adenosine A(2) receptor subtypes as well as a role for them in lacrimal gland secretion, and especially in the synergy with purinergic and cholinergic stimulation.

Place, publisher, year, edition, pages
Informa Healthcare, 2010. Vol. 35, no 6, 466-474 p.
National Category
Biological Sciences
Research subject
Biomedical Science
Identifiers
URN: urn:nbn:se:kau:diva-11580DOI: 10.3109/02713681003602667ISI: 000282896300004PubMedID: 20465439OAI: oai:DiVA.org:kau-11580DiVA: diva2:493134
Available from: 2012-02-08 Created: 2012-02-08 Last updated: 2016-02-17Bibliographically approved

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Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/pubmed/20465439http://informahealthcare.com.ezproxy.ub.gu.se/doi/pdf/10.3109/02713681003602667

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CiteExportLink to record
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