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The C subunit of Ideonella dechloratans chlorate reductase: Expression, purification, refolding, and heme reconstitution
Karlstad University, Division for Chemistry.
Karlstad University, Division for Chemistry.
2005 (English)In: Protein Expression and Purification, ISSN 1046-5928, E-ISSN 1096-0279, Vol. 41, no 2, 306-312 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2005. Vol. 41, no 2, 306-312 p.
Keyword [en]
Ideonella dechloratans; Chlorate; Chlorate reductase; ClrC; Heme; Expression; Reconstitution
Identifiers
URN: urn:nbn:se:kau:diva-2799DOI: 10.1016/j.pep.2005.02.002OAI: oai:DiVA.org:kau-2799DiVA: diva2:37547
Available from: 2008-10-07 Created: 2008-10-07 Last updated: 2010-06-10Bibliographically approved
In thesis
1. Enzymes and electron transport in microbial chlorate respiration
Open this publication in new window or tab >>Enzymes and electron transport in microbial chlorate respiration
2008 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

Microbial chlorate respiration plays an important role in the turnover of oxochlorates in nature and industrial waste management. This thesis deals with the characterization of the molecular components of chlorate respiration in Ideonella dechloratans. Chlorate respiration utilizes two soluble periplasmic enzymes, chlorate reductase and chlorite dismutase, to convert chlorate to chloride and oxygen. The genes encoding the enzymes participating in the chlorate degradation have been sequenced, and are found in close proximity, forming a gene cluster for chlorate metabolism. This work also includes the successful recombinant expression of three genes from Ideonella dechloratans. Two of the gene products, chlorite dismutase and the C subunit of chlorate reductase, participate in the chlorate respiration. The third gene, which is found close to the gene cluster for chlorate metabolism, encodes a soluble c-type cytochrome. The localization of the gene suggests the corresponding protein as a candidate for a role as electron donor to chlorate reductase. Also, the role of soluble periplasmic c cytochromes of Ideonella dechloratans in chlorate respiration was studied. At least one of the soluble c cytochromes was found capable of serving as electron donor for chlorate reduction. This c cytochrome, and several others, can also donate electrons to a terminal oxidase for subsequent reduction of oxygen, as required for the branched electron flow during chlorate respiration.

Place, publisher, year, edition, pages
Karlstad: Karlstad University, 2008. 36 p.
Series
Karlstad University Studies, ISSN 1403-8099 ; 2008:36
Keyword
Ideonella dechloratans, c cytochromes, chlorate, chlorate reductase, chlorite dismutase, heterologous expression, heme reconstitution, electron transport, oxidoreductas
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-2805 (URN)978-91-7063-196-2 (ISBN)
Public defence
2008-10-17, 9C 204, Ericssonsalen, Karlstads universitet, Karlstad, 10:30 (Swedish)
Opponent
Supervisors
Available from: 2008-10-13 Created: 2008-10-08 Last updated: 2011-11-10Bibliographically approved

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