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Rapid affinity chromatographic isolation method for LDL in human plasma by immobilized chondroitin-6-sulfate and anti-apoB-100 antibody monolithic disks in tandem
University Helsinki, Finland.
University Helsinki, Finland.ORCID iD: 0000-0002-8717-8842
Biomedicum 2U, Finland.
Biomedicum 2U, Finland.
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2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 11235Article in journal (Refereed) Published
Abstract [en]

Low-density lipoprotein (LDL) is considered the major risk factor for the development of atherosclerotic cardiovascular diseases (ASCVDs). A novel and rapid method for the isolation of LDL from human plasma was developed utilising affinity chromatography with monolithic stationary supports. The isolation method consisted of two polymeric monolithic disk columns, one immobilized with chondroitin-6-sulfate (C6S) and the other with apolipoprotein B-100 monoclonal antibody (anti-apoB-100 mAb). The first disk with C6S was targeted to remove chylomicrons, very-low-density lipoprotein (VLDL) particles, and their remnants including intermediate-density lipoprotein (IDL) particles, thus allowing the remaining major lipoprotein species, i.e. LDL, lipoprotein(a) (Lp(a)), and high-density lipoprotein (HDL) to flow to the anti-apoB-100 disk. The second disk captured LDL particles via the anti-apoB-100 mAb attached on the disk surface in a highly specific manner, permitting the selective LDL isolation. The success of LDL isolation was confirmed by different techniques including quartz crystal microbalance. In addition, the method developed gave comparable results with ultracentrifugation, conventionally used as a standard method. The reliable results achieved together with a short isolation time (less than 30 min) suggest the method to be suitable for clinically relevant LDL functional assays.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019. Vol. 9, article id 11235
Keywords [en]
IMMUNOAFFINITY CHROMATOGRAPHY; FAST SEPARATION; LIPOPROTEINS; INSIGHTS; COLUMNS
National Category
Engineering and Technology
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-74335DOI: 10.1038/s41598-019-47750-zISI: 000478575000022PubMedID: 31375727OAI: oai:DiVA.org:kau-74335DiVA, id: diva2:1342998
Available from: 2019-08-15 Created: 2019-08-15 Last updated: 2019-08-16Bibliographically approved

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Forssén, PatrikFornstedt, Torgny

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Multia, EvgenForssén, PatrikFornstedt, TorgnyOorni, Katariina
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