Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Impact of assay temperature on antibody binding characteristics in living cells: A case study
Ridgeview Instruments AB Uppsala.ORCID iD: 0000-0002-4509-4106
Charles Univ. Prague Czech Republic.
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences (from 2013).ORCID iD: 0000-0002-7123-2066
Ridgeview Instruments AB Uppsala; Uppsala universitet.
2017 (English)In: BIiomedical reports, ISSN 2049-9434, Vol. 7, no 5, p. 400-406Article in journal (Refereed) Published
Abstract [en]

Kinetic and thermodynamic studies of ligand-receptor interactions are essential for increasing the understanding of receptor activation mechanisms and drug behavior. The characterization of molecular interactions on living cells in real-time goes beyond most current binding assays, and provides valuable information about the dynamics and underlying mechanism of the molecules in a living system. The effect of temperature on interactions in cell-based assays is, however, rarely discussed. In the present study, the effect of temperature on binding of monoclonal antibodies, cetuximab and pertuzumab to specific receptors on living cancer cells was evaluated, and the affinity and kinetics of the interactions were estimated at selected key temperatures. Changes in the behavior of the interactions, particularly in the on- and off-rates were observed, leading to greatly extended time to reach the equilibrium at 21 degrees C compared with at 37 degrees C. However, the observed changes in kinetic characteristics were less than a factor of 10. It was concluded that it is possible to conduct real-time measurements with living cells at different temperatures, and demonstrated that influences of the ambient temperature on the interaction behavior are likely to be less than one order of magnitude.

Place, publisher, year, edition, pages
2017. Vol. 7, no 5, p. 400-406
National Category
Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:kau:diva-65945DOI: 10.3892/br.2017.982ISI: 000417416000002PubMedID: 29181152OAI: oai:DiVA.org:kau-65945DiVA, id: diva2:1177654
Available from: 2018-01-25 Created: 2018-01-25 Last updated: 2020-01-15Bibliographically approved

Open Access in DiVA

fulltext(402 kB)5 downloads
File information
File name FULLTEXT01.pdfFile size 402 kBChecksum SHA-512
b8f308763ada9a31eaf0fba9ea8c9f5aa913b8fafcecf8842d4b0661e74b25d2420620f1068511777335f1f9ad9847493640a1ec21faeee72a2fab665eee2b7d
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Fornstedt, Torgny

Search in DiVA

By author/editor
Encarnacao, Joao CrispimFornstedt, Torgny
By organisation
Department of Engineering and Chemical Sciences (from 2013)
Pharmaceutical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 5 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 16 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf