Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Estimation of Nonlinear Adsorption Isotherms in Gradient Elution RP-LC of Peptides in the Presence of an Adsorbing Additive
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.ORCID iD: 0000-0001-8561-6872
Show others and affiliations
2017 (English)In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 80, no 6, 961-966 p.Article in journal (Refereed) Published
Abstract [en]

In electrostatic repulsive interaction chromatography, using a charged surface hybrid sorbent carrying positive charges can improve the peak shape of peptides in reversed-phase liquid chromatography (RP-LC), especially in overloaded conditions, compared with standard C-18 sorbents. However, the positive surface charges can interact with anionic additives commonly used in peptide separations, e.g., trifluoroacetic acid (TFA), complicating adsorption isotherm estimation. We investigated how the competition for available adsorption sites between TFA and two peptides influenced the adsorption isotherm in gradient elution. A model accounting for the competition with TFA was compared with a model neglecting TFA adsorption. We found that the two models predicted elution profiles with the same accuracy. We also found that the adsorption isotherms were extremely similar in shape, leading to the conclusion that neglecting the competition with TFA is a valid approximation enabling faster and more robust adsorption isotherm estimation for the studied type of sorbent.

Place, publisher, year, edition, pages
2017. Vol. 80, no 6, 961-966 p.
Identifiers
URN: urn:nbn:se:kau:diva-65567DOI: 10.1007/s10337-017-3298-yISI: 000402824400014PubMedID: 28725083OAI: oai:DiVA.org:kau-65567DiVA: diva2:1172996
Available from: 2018-01-11 Created: 2018-01-11 Last updated: 2018-01-11

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Åsberg, Dennis

Search in DiVA

By author/editor
Åsberg, DennisFornstedt, Torgny
By organisation
Department of Engineering and Chemical Sciences
In the same journal
Chromatographia

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf