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Systematic investigations of peak deformations due to co-solvent adsorption in preparative supercritical fluid chromatography
Karlstad Univ, Dept Engn & Chem Sci, SE-65188 Karlstad, Sweden..
AstraZeneca R&D, Innovat Med & Early Dev, Resp Inflammat & Autoimmun, S-43183 Molndal, Sweden..
AstraZeneca R&D, Innovat Med & Early Dev, Resp Inflammat & Autoimmun, S-43183 Molndal, Sweden..
Karlstad Univ, Dept Engn & Chem Sci, SE-65188 Karlstad, Sweden..
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2017 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1496, p. 141-149Article in journal (Refereed) Published
Abstract [en]

Strangely shaped overloaded bands were recently reported using a standard supercritical fluid chromatographic system comprising a diol column as the stationary phase and carbon dioxide with methanol as the mobile phase, Some of these overloaded elution profiles appeared strongly deformed and even had "anti-Langmuirian" shapes although their solute compounds had "Langmuirian" adsorption. To obtain a more complete understanding of the generality of these effects, the investigation was expanded to cover also other common co-solvents, such as ethanol, 2-propanol, and acetonitrile, as well as various stationary phase materials, such as silica, and 2-ethylpyridine. From this expanded study it could be confirmed that the effects of deformed overloaded solute band shapes, due to co-solvent adsorption, is general phenomena in supercritical fluid chromatographic. It could also be concluded that these effects as well as previously observed "solvent effects" or "plug effects" are entirely due to competition between the solute and solvent molecules for the adsorption sites on the stationary phase surface. Finally, guidelines were given for how to evaluate the risk of deformations occurring for a given solvent-column combination, based simply on testing retention times of solutes and co-solvent. (C) 2017 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2017. Vol. 1496, p. 141-149
Keywords [en]
Supercritical fluid chromatography, Co-solvent adsorption, Adsorption strength, Langmuir band shape, Anti-Langmuir band shape
Identifiers
URN: urn:nbn:se:kau:diva-65479DOI: 10.1016/j.chroma.2017.03.053ISI: 000400222200016PubMedID: 28366564OAI: oai:DiVA.org:kau-65479DiVA, id: diva2:1169749
Conference
16th International Symposium on Preparative and Industrial Chromatography and Allied Techniques, OCT 09-12, 2016, Vienna, AUSTRIA
Available from: 2017-12-29 Created: 2017-12-29 Last updated: 2017-12-29

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