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High average brightness water window source for short-exposure cryomicroscopy
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2012 (English)In: Optics Letters, ISSN 0146-9592, E-ISSN 1539-4794, Vol. 37, no 21, 4425-4427 p.Article in journal (Refereed) Published
Abstract [en]

Laboratory water window cryomicroscopy has recently demonstrated similar image quality as synchrotron-based microscopy but still with much longer exposure times, prohibiting the spread to a wider scientific community. Here we demonstrate high-resolution laboratory water window imaging of cryofrozen cells with 10 s range exposure times. The major improvement is the operation of a lambda = 2.48 nm, 2 kHz liquid nitrogen jet laser plasma source with high spatial and temporal stability at high average brightness >1.5 x 10(12) ph/(s x sr x mu m(2) x line), i.e., close to that of early synchrotrons. Thus, this source enables not only biological x-ray microscopy in the home laboratory but potentially other applications previously only accessible at synchrotron facilities.

Place, publisher, year, edition, pages
Optical Society of America, 2012. Vol. 37, no 21, 4425-4427 p.
Keyword [en]
X-Ray Microscopy
National Category
Other Physics Topics
Research subject
Physics
Identifiers
URN: urn:nbn:se:kau:diva-63911DOI: 10.1364/OL.37.004425ISI: 000310577700024Scopus ID: 2-s2.0-84868308552OAI: oai:DiVA.org:kau-63911DiVA: diva2:1143794
Funder
Swedish Research CouncilSwedish Foundation for Strategic Research
Available from: 2017-09-22 Created: 2017-09-22 Last updated: 2017-10-05Bibliographically approved

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Selin, MårtenHolmberg, AndersVogt, UlrichHertz, Hans M.
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Citation style
  • apa
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  • de-DE
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