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Viscosity contrast effects in analytical scale chromatography: Evidence and impact
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.ORCID iD: 0000-0003-1819-1709
Univ Western Sydney, Sch Sci & Hlth, Australian Ctr Res Separat Sci, North Parramatta, NSW 2751, Australia..
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.
2017 (English)In: Microchemical journal (Print), ISSN 0026-265X, E-ISSN 1095-9149, Vol. 130, 102-107 p.Article in journal (Refereed) Published
Abstract [en]

The phenomenon known as viscous fingering has been shown to be very detrimental to separation performance in preparative and size exclusion chromatography, and also in multidimensional HPLC However, there are few reports of viscous fingering in analytical scale high performance liquid chromatography (HPLC), despite samples very often being introduced to the analytical-size HPLC columns in a solvent have a substantially different viscosity than the mobile phase. With this study we aim at first hand to investigate if viscous fingering or any similar and related effects to these in preparative levels also take place in analytical scale HPLC and if so, what impact this have on the separation performance. We could show that not only viscous finger does occur in analytical scale columns but also that peak distortions are apparent already at viscosity ratios between eluent and sample solution approaching unity. The latter indicates that a pre-viscous phenomenon is occurring that could be more important than the viscous fingering itself at the analytical format. As the viscosity contrast increases, the leading edge of the sample band distorts and the band volume increases, both leading to a decrease in performance.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 130, 102-107 p.
Keyword [en]
Analytical scale HPLC, Viscous fingering, Viscosity contrast, Column efficiency
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-62621DOI: 10.1016/j.microc.2016.08.007ISI: 000390072400015OAI: oai:DiVA.org:kau-62621DiVA: diva2:1130706
Available from: 2017-08-10 Created: 2017-08-10 Last updated: 2017-08-10Bibliographically approved

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