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Försök att identifiera ett NapC/NirT -protein hos Ideonella dechloratans - en tänkbar elektrondonator till periplasmatiskt kloratreduktas
2004 (Swedish)Independent thesis Basic level (professional degree)Student thesis
Abstract [en]

The aim with this work was to probe the presence of membrane anchored protein in Ideonella dechloratans, with the function of donating electrons to the periplasmatic chlorate reductase. The project has two parts; one genetic and one protein chemical. In the genetic part I tried to identify a gene for a NapC/NirT-like protein by touchdown PCR, using degenerated primers. The gene was not found. In the protein chemical part, cell membrane proteins were separated by SDS-PAGE, followed by staining with tetramethylbenzidine (TMBZ) for covalently bound heme. The result was five different proteins with molecular weights between 13-35,5 kDa, and also a strongly indication that I. dechloratans use cbb3 as terminal oxidase for reduction of oxygen under aerobic circumstances. The molecular weights of the separated proteins correspond to those expected for a cbb3 oxidase. I also attempted to extract the received proteins from the polyacrylamide gel. The result was satisfactory.

Place, publisher, year, edition, pages
2004. , 40 p.
Identifiers
URN: urn:nbn:se:kau:diva-52546Local ID: KEM-22OAI: oai:DiVA.org:kau-52546DiVA: diva2:1101073
Subject / course
Chemistry
Available from: 2017-05-29 Created: 2017-05-29

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