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Surface enhanced Raman spectroscopy within pharmaceutical developement
2002 (English)Independent thesis Advanced level (degree of Master (One Year))Student thesis
Abstract [en]

Raman spectroscopy offers great selectivity and the Raman spectrum is rich of information, although the method suffers from poor detection limits. In surface enhanced Raman spectroscopy (SERS) the signal to noise ratio can be greatly increased. In this study the usefulness of SERS within pharmaceutical development is evaluated through investigating and identifying the parameters that are of importance for obtaining the SERS effect. The reproducibility of the SERS signal and the enhancement factors were studied. A range of different substances have been used as model compounds. The compounds were chosen based on their pharmaceutical interest and the pKa value. Different modifications of the original colloid sol preparation method proposed by Lee and Meisel were evaluated as SERS substrates along with a commercially available SERS matrix. The conclusion that could be drawn from the different modification of the colloid sol method was that boiling the sol under reflux gave the best reproducibility. The SERS study of the different compounds was carried out at several adsorbate concentrations and it was concluded that the enhancement factor increased with a decrease in concentration. It was also noticed that initiated aggregation of the sol was an indication of an activation of the sol leading to enhancement. The commercially available sol coated vials did not live up to expectations. Only two of the compound investigated gave an enhancement in these vials and the results obtained were difficult to evaluate since the coating of the vials gave a background that interfered with the spectrum of the analyte. A new method where the SERS sample (colloid sol with analyte) was measured in levitated drops was also endeavoured. The enhancement of the signal in this method has probably to do with two effects, the original enhancement mechanisms and an increased aggregation rate in the SERS droplet. The increased aggregation rate leads to a more active colloid sol that in turn increases the enhancement.

Place, publisher, year, edition, pages
2002. , 55 p.
Identifiers
URN: urn:nbn:se:kau:diva-52472Local ID: KEM-15OAI: oai:DiVA.org:kau-52472DiVA: diva2:1100998
Subject / course
Chemistry
Available from: 2017-05-29 Created: 2017-05-29

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Citation style
  • apa
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