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Stroke-induced stem cells proliferation in normal versus diabetic mice and pharmacological regulation
Karlstads universitet, Fakulteten för hälsa, natur- och teknikvetenskap (from 2013).
2015 (Engelska)Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)Alternativ titel
Stroke-inducerad stamcells proliferation i normala kontra diabetiska möss och famakologisk reglering (Svenska)
Abstract [en]

Introduction: Stroke is caused from the occlusion of any cerebral artery leading to cerebral ischemia, brain damage and consequent neurological impairments and disability. The primary causes of mortality in western populations is stroke. Diabetes type 2 is a high risk factor for stroke. Stroke leads to an observable increase of neural stem cell proliferation in the subventricular zone and enhances neurogenesis in the adult rodent and human brain which suggest a mechanism contributing to stroke recovery. Neurogenesis in type 2 diabetes patients is impaired. However, whether stroke-induced neurogenesis is impaired in diabetes has not been studied. Exendin-4 is a drug for clinical treatment of type 2 diabetes which has been shown to have neuroprotective properties in animal studies. However whether Exendine-4 leads to increased neurogenesis after  stroke in the diabetic brain has not been previously studied.

 Aims: The specific aims of this project were to determine whether stroke-induced stem cell proliferation is impacted by diabetes in the mouse, and if Exendine-4 regulates stroke-induced stem cell proliferation in normal and diabetic mice.

Material and Methods: Aged obese/type 2 diabetic mice were subjected to stroke. The Exendin-4 treatment was started 1.5 hours thereafter. Treatment was continued for one week before animals were sacrificed. Brains were isolated and the neurons were immunostained using the specific proliferation marker Ki67. Neural stem cell proliferation was quantified by counting Ki67+ cells in the ipsilateral (subventricular zone in stroke hemisphere).The estimation was assessed by stereological counts of proliferating stem cell in the subventricular zone.

 Results: The number of proliferating stem cell after stroke was statistically significantly higher in the normal mice versus diabetic mice. The effect was present in both sides (control and stroke) of the subventricular zone. Exendine-4 treatment induced statistically significant increased of  stem cell proliferation in normal mice but not in diabetic mice.

 

Conclusions: The result of this study shows that type 2 diabetes decreased the proliferation of neural stem cell in the subventricular zone and that Exendin-4 enhanced the subventricular proliferation in a preclinical model of clinical relevance. The data suggest that the Exendin-4 treatment could be administered to normal patients suffering from stroke in the ambulance or in the emergency room although more studies are needed. 

Ort, förlag, år, upplaga, sidor
2015. , s. 19
Nyckelord [en]
Stroke, diabetes, GLP-1, Exendin-4, neurogenesis, stem cell proliferation, animal model, mouse
Nationell ämneskategori
Kemiteknik
Identifikatorer
URN: urn:nbn:se:kau:diva-36776OAI: oai:DiVA.org:kau-36776DiVA, id: diva2:825963
Externt samarbete
Karolinska Institutet, Södersjukhuset
Ämne / kurs
Kemiteknik
Utbildningsprogram
Civilingenjör: Kemiteknik (300 hp)
Handledare
Examinatorer
Tillgänglig från: 2015-06-24 Skapad: 2015-06-24 Senast uppdaterad: 2015-06-24Bibliografiskt granskad

Open Access i DiVA

Zainab Fadhel- Project 30hp(248 kB)190 nedladdningar
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Fakulteten för hälsa, natur- och teknikvetenskap (from 2013)
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