Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Why ultra high performance liquid chromatography produces more tailing peaks than high performance liquid chromatography, why it does not matter and how it can be addressed
AstraZeneca R&D Lund, SE-221 87 Lund, Sweden.
Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemi och biomedicinsk vetenskap.
Department of Physical and Analytical Chemistry, BMC Box 577, SE-751 23 Uppsala, Sweden.
AstraZeneca Nordic Headquarters (ISMO), Södertälje. (, SE-151 85 Sweden)
Visa övriga samt affilieringar
2011 (Engelska)Ingår i: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 1218, nr 39, s. 6914-6921Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The purpose of this study is to demonstrate, with experiments and with computer simulations based on a firm chromatographic theory, that the wide spread perception of that the United States Pharmacopeia tailing factor must be lower than 2 (Tf < 2) is questionable when using the latest generation of LC equipment. It is shown that highly efficient LC separations like those obtained with sub-2 μm porous and 2.7 μm superficially porous particles (UHPLC) produce significantly higher Tf-values than the corresponding separation based on 3 μm porous particles (HPLC) when the same amount of sample is injected. Still UHPLC separations provide a better resolution to adjacent peaks. Expressions have been derived that describe how the Tf-value changes with particle size or number of theoretical plates. Expressions have also been derived that can be used to scale the injection volume based on particle size or number of theoretical plates to maintain the Tf-value when translating a HPLC separation to the corresponding UHPLC separation. An aspect that has been ignored in previous publications. Finally, data obtained from columns with different age/condition indicate that Tf-values should be complemented by a peak width measure to provide a more objective quality measure.

Ort, förlag, år, upplaga, sidor
2011. Vol. 1218, nr 39, s. 6914-6921
Nyckelord [en]
HPLC, Isotherm, Particle size, Simulation, Translation, UHPLC
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:kau:diva-29017DOI: 10.1016/j.chroma.2011.08.018ISI: 000295241500009OAI: oai:DiVA.org:kau-29017DiVA, id: diva2:647906
Tillgänglig från: 2013-09-12 Skapad: 2013-09-12 Senast uppdaterad: 2019-07-12Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextFull text

Personposter BETA

Forssén, PatrikFornstedt, Torgny

Sök vidare i DiVA

Av författaren/redaktören
Forssén, PatrikFornstedt, Torgny
Av organisationen
Avdelningen för kemi och biomedicinsk vetenskap
I samma tidskrift
Journal of chromatography. B
Analytisk kemi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 79 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf