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Co-immobilization of oxalate oxidase and catalase in films for scavenging of oxygen or oxalic acid
Kemiska institutionen, Department of Chemistry, Umeå Universitet.
Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemiteknik.
Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemiteknik. (Paper Surface Centre)ORCID-id: 0000-0002-1256-1708
Kemiska institutionen, Department of Chemistry, Umeå Universitet.
2013 (Engelska)Ingår i: Biochemical engineering journal, ISSN 1369-703X, E-ISSN 1873-295X, Vol. 72, s. 96-101Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Oxalate oxidase has potential to act as an oxygen scavenger in active packaging to increase the shelf-life of food and beverages, while simultaneously producing the protective packaging gas carbon dioxide. This study shows that oxalate oxidase from barley can be immobilized with retained catalytic activity through entrapment in a latex polymer matrix. Conditions for formation of film containing oxalate oxidase have been evaluated as well as effects of storage and latex on enzyme activity, migration of enzyme in films, and the ability of the latex films to resist higher temperatures. Drying of enzyme-containing latex films at 75 °C prior to conditioning at 30 °C resulted in higher activity than drying solely at 30 °C, or drying at 95 °C or 105 °C followed by conditioning at 30 °C. Storage of films in air at 4 °C for 14 days did not negatively affect the enzymatic activity. Inclusion of catalase in films with oxalate oxidase effectively prevented release of hydrogen peroxide. The results suggest that the immobilized enzyme can successfully be used both as an oxygen scavenger and as an oxalic-acid scavenger.

Ort, förlag, år, upplaga, sidor
2013. Vol. 72, s. 96-101
Nyckelord [en]
Oxalate oxidase, active packaging, oxygen scavenger, oxalic acid, latex, entrapment
Nationell ämneskategori
Industriell bioteknik Kemiteknik
Identifikatorer
URN: urn:nbn:se:kau:diva-26574DOI: 10.1016/j.bej.2013.01.006ISI: 000319373200014OAI: oai:DiVA.org:kau-26574DiVA, id: diva2:609561
Tillgänglig från: 2013-03-06 Skapad: 2013-03-06 Senast uppdaterad: 2017-12-06Bibliografiskt granskad
Ingår i avhandling
1. Oxygen-reducing enzymes in coatings and films for active packaging
Öppna denna publikation i ny flik eller fönster >>Oxygen-reducing enzymes in coatings and films for active packaging
2013 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Oxygen scavengers are used in active packages to protect the food against deteriorative oxidation processes. The aim of this work was to investigate the possibilities to produce oxygen-scavenging packaging materials based on oxygen-reducing enzymes. The enzymes were incorporated into a dispersion coating formulation applied onto a food-packaging board using conventional laboratory coating techniques.

Various enzymes were used: a glucose oxidase, an oxalate oxidase and three laccases originating from different organisms. All of the enzymes were successfully incorporated into a coating layer and could be reactivated after drying. For at least two of the enzymes, re-activation was possible not only by using liquid water but also by using water vapour. Re-activation of the glucose oxidase and a laccase required relative humidities of greater than 75% and greater than 92%, respectively.

Catalytic reduction of oxygen gas by glucose oxidase was promoted by creating an open structure through addition of clay to the coating at a level above the critical pigment volume concentration. Migration of the enzyme and the substrate was reduced by adding an extrusion-coated liner of polypropylene on top of the coating.

For the laccase-catalysed reduction of oxygen it was possible to use lignin derivatives as substrates for the enzymatic reaction. The laccase-catalysed reaction created a polymeric network by cross-linking of lignin-based entities, which resulted in increased stiffness and increased water-resistance of biopolymer films. The laccases were also investigated with regard to their potential to function as oxygen scavengers at low temperatures. At 7°C all three laccases retained more than 20% of the activity they had at room temperature (25°C), which suggests that the system is also useful for packaging of refrigerated food.

Ort, förlag, år, upplaga, sidor
Karlstad: Karlstads universitet, 2013. s. 91
Serie
Karlstad University Studies, ISSN 1403-8099 ; 2013:38
Nyckelord
Active packaging, food packaging, oxygen scavengers, oxygen-reducing enzymes, dispersion coating, biopolymers
Nationell ämneskategori
Kemiteknik
Forskningsämne
Kemiteknik
Identifikatorer
urn:nbn:se:kau:diva-28749 (URN)978-91-7063-516-8 (ISBN)
Disputation
2013-10-18, 9C204, Rejmersalen, Karlstad, 10:15 (Engelska)
Opponent
Handledare
Tillgänglig från: 2013-09-27 Skapad: 2013-08-27 Senast uppdaterad: 2014-10-27Bibliografiskt granskad

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Johansson, KristinJärnström, Lars

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Totalt: 220 träffar
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