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Optimization strategies accounting for the additive in preparative chiral liquid chromatography
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
Uppsala University.
Institute of Pharmaceutical Sciences, University of Tübingen, Germany.
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.ORCID iD: 0000-0003-1819-1709
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2012 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1269, p. 279-286, article id SIArticle in journal (Refereed) Published
Abstract [en]

This study is an in-depth investigation on how numerical optimization strategies that also account forthe additive type and concentration, in preparative batch chromatography, should be performed. As amodel system, the separation of Z-(R,S)-2-aminobutyric acid enantiomers on a quinidine carbamate-based chiral stationary phase in polar organic mode was used, with different additive strengths of aceticacid or hexanoic acid in methanol. The inverse method was used to determine the competitive adsorp-tion isotherm parameters for the enantiomers and the additives. Three different optimization strategieswere examined: (1) injection volume optimization, (2) optimization of injection volume and additiveconcentration, and (3) full optimization including injection volume, additive concentration, sample con-centration and flow rate. It was concluded that (i) it is important to incorporate the additive concentrationin the optimization procedure to achieve the highest production rates, (ii) the full optimization strategyhad the overall best results, and (iii) the selection of additive is very important (here acetic acid additivewas superior to the hexanoic acid additive). By including the additive in the adsorption model and inthe numerical optimization it is not only possible to achieve higher production rates but also to properlyselect the additive that is most advantageous for the specific separation problem.

Place, publisher, year, edition, pages
2012. Vol. 1269, p. 279-286, article id SI
Keywords [en]
Additive, Optimization, Enantiomers, Chiral preparative Chromatography, Inverse method
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-16059DOI: 10.1016/j.chroma.2012.05.052ISI: 000312475300023PubMedID: 22742895OAI: oai:DiVA.org:kau-16059DiVA, id: diva2:573659
Funder
Knowledge FoundationSwedish Research Council
Note

Research Funder: Research Council for Environment, Agricultural Sciences and Spatial Planning 

Available from: 2012-12-03 Created: 2012-12-03 Last updated: 2017-12-07Bibliographically approved

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Forssén, PatrikSamuelsson, JörgenFornstedt, Torgny

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