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New Techniques for Sample Preparation in Analytical Chemistry: Microextraction in Packed Syringe (MEPS) and Methacrylate Based Monolithic Pipette Tips
Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemi och biomedicinsk vetenskap.
2008 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Sample preparation is often a bottleneck in systems for chemical analysis. The aim of this work was to investigate and develop new techniques to address some of the shortcomings of current sample preparation methods. The goal has been to provide full automation, on-line coupling to detection systems, short sample preparation times and high-throughput.

In this work a new technique for sample preparation that can be connected on-line to liquid chromatography (LC) and gas chromatography (GC) has been developed. Microextraction in packed syringe (MEPS) is a new solid-phase extraction (SPE) technique that is miniaturized and can be fully automated. In MEPS approximately 1 mg of sorbent material is inserted into a gas tight syringe (100-250 μL) as a plug. Sample preparation takes place on the packed bed. Evaluation of the technique was done by the determination of local anaesthetics in human plasma samples using MEPS on-line with LC and tandem mass spectrometry (MS-MS). MEPS connected to an autosampler was fully automated and clean-up of the samples took about one minute. In addition, in the case of plasma samples the same plug of sorbent could be used for about 100 extractions before it was discarded.

A further aim of this work was to increase sample preparation throughput. To do that disposable pipette tips were packed with a plug of porous polymer monoliths as sample adsorbent and were then used in connection with 96-well plates and LC-MS-MS. The evaluation of the methods was done by the analysis of local anaesthetics lidocaine and ropivacaine, and anti-cancer drug roscovitine in plasma samples. When roscovitine and lidocaine in human plasma and water samples were used as model substances, a 96-plate was handled in about two minutes. Further, disposable pipette tips may be produced at low cost and because they are used only once, carry-over is eliminated.

Ort, förlag, år, upplaga, sidor
Fakulteten för teknik- och naturvetenskap , 2008. , s. 66
Serie
Karlstad University Studies, ISSN 1403-8099 ; 2008:4
Nyckelord [en]
Liquid Chromatography/Tandem Mass Spectrometry, Local Anaesthetics, Microextraction in Packed Syringe, Monoliths, Pipette Tips, Sample Preparation
Nationell ämneskategori
Analytisk kemi
Forskningsämne
Kemi
Identifikatorer
URN: urn:nbn:se:kau:diva-1420ISBN: 978-91-7063-161-0 (tryckt)OAI: oai:DiVA.org:kau-1420DiVA, id: diva2:5312
Disputation
2008-02-22, 9C204, Universitetsgatan 2, 651 88, Karlstad, 10:15
Opponent
Handledare
Tillgänglig från: 2008-02-12 Skapad: 2008-02-12 Senast uppdaterad: 2011-11-09
Delarbeten
1. Microextraction in packed syringe (MEPS) for liquid and gas chromatographic applications: Part II - Determination of ropivacaine and its metabolites in human plasma samples using MEPS with liquid chromatography/tandem mass spectrometry
Öppna denna publikation i ny flik eller fönster >>Microextraction in packed syringe (MEPS) for liquid and gas chromatographic applications: Part II - Determination of ropivacaine and its metabolites in human plasma samples using MEPS with liquid chromatography/tandem mass spectrometry
2004 (Engelska)Ingår i: Journal of Mass Spectrometry, ISSN 1076-5174, E-ISSN 1096-9888, Vol. 39, nr 12, s. 1488-1493Artikel i tidskrift (Refereegranskat) Published
Identifikatorer
urn:nbn:se:kau:diva-1991 (URN)10.1002/jms.731 (DOI)
Tillgänglig från: 2008-02-12 Skapad: 2008-02-12 Senast uppdaterad: 2017-12-07Bibliografiskt granskad
2. New trends in sample preparation: on-line microextraction in packed syringe (MEPS) for LC and GC applications Part III: Determination and validation of local anaesthetics in human plasma samples using a cation-exchange sorbent, and MEPS–LC–MS–MS
Öppna denna publikation i ny flik eller fönster >>New trends in sample preparation: on-line microextraction in packed syringe (MEPS) for LC and GC applications Part III: Determination and validation of local anaesthetics in human plasma samples using a cation-exchange sorbent, and MEPS–LC–MS–MS
2004 (Engelska)Ingår i: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 813, nr 1-2, s. 129-135Artikel i tidskrift (Refereegranskat) Published
Identifikatorer
urn:nbn:se:kau:diva-1972 (URN)10.1016/j.jchromb.2004.09.020 (DOI)
Tillgänglig från: 2008-02-05 Skapad: 2008-02-05 Senast uppdaterad: 2017-12-07Bibliografiskt granskad
3. Increasing Sample Preparation Throughput Using Monolithic Methacrylate Polymer as Packing Material for 96-Tip Robotic Device
Öppna denna publikation i ny flik eller fönster >>Increasing Sample Preparation Throughput Using Monolithic Methacrylate Polymer as Packing Material for 96-Tip Robotic Device
2006 (Engelska)Ingår i: Journal of Liquid Chromatography & Related Technologies, ISSN 1082-6076, E-ISSN 1520-572X, Vol. 29, nr 10, s. 1477-1489Artikel i tidskrift (Refereegranskat) Published
Identifikatorer
urn:nbn:se:kau:diva-1993 (URN)10.1080/10826070600674919 (DOI)
Tillgänglig från: 2008-02-12 Skapad: 2008-02-12 Senast uppdaterad: 2017-12-07Bibliografiskt granskad
4. Surface modified polypropylene pipette tips packed with a monolithic plug of adsorbent for high-throughput sample preparation
Öppna denna publikation i ny flik eller fönster >>Surface modified polypropylene pipette tips packed with a monolithic plug of adsorbent for high-throughput sample preparation
2007 (Engelska)Ingår i: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 30, nr 12, s. 1964-1972Artikel i tidskrift (Refereegranskat) Published
Identifikatorer
urn:nbn:se:kau:diva-1994 (URN)10.1002/jssc.200600523 (DOI)
Tillgänglig från: 2008-02-12 Skapad: 2008-02-12 Senast uppdaterad: 2017-12-07Bibliografiskt granskad
5. Some Factors Affecting the Performance of Microextraction in Packed Syringe (MEPS)
Öppna denna publikation i ny flik eller fönster >>Some Factors Affecting the Performance of Microextraction in Packed Syringe (MEPS)
(Engelska)Manuskript (Övrigt vetenskapligt)
Nationell ämneskategori
Analytisk kemi
Forskningsämne
Kemi
Identifikatorer
urn:nbn:se:kau:diva-1995 (URN)
Tillgänglig från: 2008-02-12 Skapad: 2008-02-12 Senast uppdaterad: 2011-11-09Bibliografiskt granskad

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