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Microextraction in packed syringe (MEPS) for liquid and gas chromatographic applications: Part II - Determination of ropivacaine and its metabolites in human plasma samples using MEPS with liquid chromatography/tandem mass spectrometry
Karlstads universitet, Institutionen för kemi.
Karlstads universitet, Institutionen för kemi.
Karlstads universitet, Institutionen för kemi.
2004 (Engelska)Ingår i: Journal of Mass Spectrometry, ISSN 1076-5174, E-ISSN 1096-9888, Vol. 39, nr 12, s. 1488-1493Artikel i tidskrift (Refereegranskat) Published
Ort, förlag, år, upplaga, sidor
2004. Vol. 39, nr 12, s. 1488-1493
Identifikatorer
URN: urn:nbn:se:kau:diva-1991DOI: 10.1002/jms.731OAI: oai:DiVA.org:kau-1991DiVA, id: diva2:5307
Tillgänglig från: 2008-02-12 Skapad: 2008-02-12 Senast uppdaterad: 2017-12-07Bibliografiskt granskad
Ingår i avhandling
1. New Techniques for Sample Preparation in Analytical Chemistry: Microextraction in Packed Syringe (MEPS) and Methacrylate Based Monolithic Pipette Tips
Öppna denna publikation i ny flik eller fönster >>New Techniques for Sample Preparation in Analytical Chemistry: Microextraction in Packed Syringe (MEPS) and Methacrylate Based Monolithic Pipette Tips
2008 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Sample preparation is often a bottleneck in systems for chemical analysis. The aim of this work was to investigate and develop new techniques to address some of the shortcomings of current sample preparation methods. The goal has been to provide full automation, on-line coupling to detection systems, short sample preparation times and high-throughput.

In this work a new technique for sample preparation that can be connected on-line to liquid chromatography (LC) and gas chromatography (GC) has been developed. Microextraction in packed syringe (MEPS) is a new solid-phase extraction (SPE) technique that is miniaturized and can be fully automated. In MEPS approximately 1 mg of sorbent material is inserted into a gas tight syringe (100-250 μL) as a plug. Sample preparation takes place on the packed bed. Evaluation of the technique was done by the determination of local anaesthetics in human plasma samples using MEPS on-line with LC and tandem mass spectrometry (MS-MS). MEPS connected to an autosampler was fully automated and clean-up of the samples took about one minute. In addition, in the case of plasma samples the same plug of sorbent could be used for about 100 extractions before it was discarded.

A further aim of this work was to increase sample preparation throughput. To do that disposable pipette tips were packed with a plug of porous polymer monoliths as sample adsorbent and were then used in connection with 96-well plates and LC-MS-MS. The evaluation of the methods was done by the analysis of local anaesthetics lidocaine and ropivacaine, and anti-cancer drug roscovitine in plasma samples. When roscovitine and lidocaine in human plasma and water samples were used as model substances, a 96-plate was handled in about two minutes. Further, disposable pipette tips may be produced at low cost and because they are used only once, carry-over is eliminated.

Ort, förlag, år, upplaga, sidor
Fakulteten för teknik- och naturvetenskap, 2008. s. 66
Serie
Karlstad University Studies, ISSN 1403-8099 ; 2008:4
Nyckelord
Liquid Chromatography/Tandem Mass Spectrometry, Local Anaesthetics, Microextraction in Packed Syringe, Monoliths, Pipette Tips, Sample Preparation
Nationell ämneskategori
Analytisk kemi
Forskningsämne
Kemi
Identifikatorer
urn:nbn:se:kau:diva-1420 (URN)978-91-7063-161-0 (ISBN)
Disputation
2008-02-22, 9C204, Universitetsgatan 2, 651 88, Karlstad, 10:15
Opponent
Handledare
Tillgänglig från: 2008-02-12 Skapad: 2008-02-12 Senast uppdaterad: 2011-11-09
2. New Techniques for Sample Preparation in Analytical Chemistry
Öppna denna publikation i ny flik eller fönster >>New Techniques for Sample Preparation in Analytical Chemistry
2005 (Engelska)Licentiatavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Sample preparation is often a bottleneck in systems for chemical analysis. The aim of this work was to investigate and develop new techniques to address some of the shortcomings of current sample preparation methods. The goal has been to provide full automation, on-line coupling to detection systems, short sample preparation times and high-throughput.

A new technique for sample preparation that can be connected on-line to liquid chromatography (LC) and gas chromatography (GC) has been developed. Microextraction in packed syringe (MEPS) is a new solid-phase extraction (SPE) technique that is miniaturized and can be fully automated. In MEPS approximately 1 mg of sorbent material is inserted into a gas tight syringe (100-250 μL) as a plug. Sample preparation takes place on the packed bed. Evaluation of the technique was done by the determination of local anaesthetics in human plasma samples using MEPS on-line with LC and tandem mass spectrometry (MS-MS). MEPS connected to an autosampler was fully automated and clean-up of the samples took one minute. In addition, in the case of plasma samples the same plug of sorbent could be used for about 100 extractions before it was discarded.

A further aim of this work was to increase sample preparation throughput. To do that disposable pipette tips were packed with a plug of porous polymer monoliths as sample adsorbent and were then used in connection with 96-well plates and LC-MS-MS. When roscovitine in human plasma and water samples was used as model substance, a 96-plate was handled in two minutes.

Ort, förlag, år, upplaga, sidor
Institutionen för kemi, 2005
Serie
Karlstad University Studies, ISSN 1403-8099 ; 2005:24
Nationell ämneskategori
Kemi
Forskningsämne
Kemi
Identifikatorer
urn:nbn:se:kau:diva-1374 (URN)91-85335-65-7 (ISBN)
Presentation
2005-06-15, Ericssonsalen, 9C 204, Karlstads universitet, Karlstad, 10:15
Tillgänglig från: 2008-02-05 Skapad: 2008-02-05 Senast uppdaterad: 2010-09-14

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