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Expression of Chlorite Dismutase and Chlorate Reductase in the Prescence of Oxygen and/or Chlorate as the Terminal Electron Acceptor in Ideonella dechloratans
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences. (Skogsindustriell förädling och energiteknik)
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences. (Skogsindustriell förädling och energiteknik)
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.ORCID iD: 0000-0002-0024-5302
2012 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 78, no 12, p. 4380-4385Article in journal (Refereed) Published
Abstract [en]

The ability of microorganisms to perform dissimilatory (per)chlorate reduction is, for most species, known to be oxygen sensitive. Consequently, bioremediation processes for the removal of oxochlorates will be disturbed if oxygen is present. We measured the expression of chlorite dismutase and chlorate reductase in the presence of different terminal electron acceptors in the chlorate reducer Ideonella dechloratans. Enzyme activity assays and mRNA analyses by real-time quantitative reverse transcription (qRT)-PCR were performed on cell extracts from cells grown aerobically with and without chlorate and on cells grown anaerobically in the presence of chlorate. Our results showed that both chlorite dismutase and chlorate reductase are expressed during aerobic growth. However, transfer to anaerobic conditions with chlorate resulted in significantly enhanced enzyme activities and mRNA levels for both enzymes. Absence of oxygen was necessary for the induction to occur, since chlorate addition under aerobic conditions produced neither increased enzyme activities nor higher relative levels of mRNA. For chlorite dismutase, the observed increase in activity was on the same order of magnitude as the increase in the relative mRNA level, indicating gene regulation at the transcriptional level. However, chlorate reductase showed about 200 times higher enzyme activity in anaerobically induced cells, whereas the increase in mRNA was only about 10-fold, suggesting additional mechanisms influence the enzyme activity.

Place, publisher, year, edition, pages
American Society for Microbiology , 2012. Vol. 78, no 12, p. 4380-4385
National Category
Chemical Sciences Microbiology
Identifiers
URN: urn:nbn:se:kau:diva-13406DOI: 10.1128/AEM.07303-11ISI: 000304788500042OAI: oai:DiVA.org:kau-13406DiVA, id: diva2:529189
Available from: 2012-05-29 Created: 2012-05-29 Last updated: 2018-07-18Bibliographically approved
In thesis
1. Oxygen-dependent regulation of key components in microbial chlorate respiration
Open this publication in new window or tab >>Oxygen-dependent regulation of key components in microbial chlorate respiration
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Contamination of perchlorate and chlorate in nature is primarily the result of various industrial processes. The microbial respiration of these oxyanions of chlorine plays a major role in reducing the society’s impact on the environment. The focus with this thesis is to investigate the oxygen-dependent regulation of key components involved in the chlorate respiration in the gram‑negative bacterium Ideonella dechloratans. Chlorate metabolism is based on the action of the enzymes chlorate reductase and chlorite dismutase and results in the end products molecular oxygen and chloride ion. Up‑regulation of chlorite dismutase activity in the absence of oxygen is demonstrated to occur at the transcriptional level, with the participation of the transcriptional fumarate and nitrate reduction regulator (FNR). Also, the chlorate reductase enzyme was shown to be regulated at the transcriptional level with the possible involvement of additional regulating mechanisms as well. Interestingly, the corresponding chlorate reductase operon was found to be part of a polycistronic mRNA which also comprises the gene for a cytochrome c and a putative transcriptional regulator protein.

Place, publisher, year, edition, pages
Karlstad: Karlstads universitet, 2016
Series
Karlstad University Studies, ISSN 1403-8099 ; 13
Keywords
Anaerobic respiration, Gene expression, Chlorate, Chlorate reductase, Chlorite dismutase
National Category
Chemical Engineering
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-40698 (URN)978-91-7063-692-9 (ISBN)
Public defence
2016-04-08, Rejmersalen, 9C204, Karlstad, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2016-03-18 Created: 2016-02-25 Last updated: 2016-04-20Bibliographically approved

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Hellberg Lindqvist, MiriamNilsson, ThomasRova, Maria

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