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Characterization of a cytochrome c gene located at the gene cluster for chlorate respiration in Ideonella dechloratans
Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemi och biomedicinsk vetenskap.
Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemi och biomedicinsk vetenskap.
Novozymes Biopharma AB, Lund, Sweden.
Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemi och biomedicinsk vetenskap.
Vise andre og tillknytning
2010 (engelsk)Inngår i: Microbiology Research, ISSN 0944-5013, E-ISSN 1618-0623, Vol. 165, s. 450-457Artikkel i tidsskrift (Fagfellevurdert) Published
sted, utgiver, år, opplag, sider
2010. Vol. 165, s. 450-457
HSV kategori
Forskningsprogram
Kemi
Identifikatorer
URN: urn:nbn:se:kau:diva-8162DOI: 10.1016/j.micres.2009.10.003ISI: 000282633000002PubMedID: 20015627OAI: oai:DiVA.org:kau-8162DiVA, id: diva2:439189
Tilgjengelig fra: 2011-09-06 Laget: 2011-09-06 Sist oppdatert: 2018-01-23bibliografisk kontrollert
Inngår i avhandling
1. c Cytochromes as Electron Carriers in Microbial Chlorate Respiration
Åpne denne publikasjonen i ny fane eller vindu >>c Cytochromes as Electron Carriers in Microbial Chlorate Respiration
2011 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Microbial respiration of oxochlorates is important for the biotreatment of effluents from industries where oxochlorates are produced or handled. Several bacterial species are capable to use perchlorate and/or chlorate as an alternative electron acceptor in absence of oxygen. The present study deals with the electron transport from the membrane-bound components to the periplasmic chlorate reductase, in the gram-negative bacterium Ideonella dechloratans. Both chlorate reductase and the terminal oxidase of I. dechloratans were found to utilize soluble c cytochromes as electron donors. For further investigation, two major heme-containing components were purified and characterized. The most abundant was a 9 kDa c-type cytochrome (class I), denoted cytochrome c-Id1. This protein was shown to serve as electron donor for both chlorate reductase, and for a terminal oxidase. The other major component was a 55 kDa homotetrameric cytochrome c', (class II). A function for this cytochrome could not be demonstrated but it does not appear to serve as electron donor to chlorate reductase. A gene predicted to encode a soluble c cytochrome was found in close proximity to the gene cluster for chlorate reduction. The predicted sequence did not match any of the cytochromes discussed above. The gene was cloned and expressed heterologously, and the resulting protein was investigated as a candidate electron donor for chlorate reductase. Electron transfer from this protein could not be demonstrated, suggesting that the gene product does not serve as immediate electron donor for chlorate reductase.

sted, utgiver, år, opplag, sider
Karlstad: Karlstad University, 2011. s. 48
Serie
Karlstad University Studies, ISSN 1403-8099 ; 2011:40
Emneord
c cytochrome, chlorate reduction, electron transport
HSV kategori
Forskningsprogram
Kemi
Identifikatorer
urn:nbn:se:kau:diva-8038 (URN)978-91-7063-375-1 (ISBN)
Disputas
2011-09-30, Andersalen, 11D 121, Karlstads universitet, Karlstad, 10:15 (svensk)
Opponent
Veileder
Tilgjengelig fra: 2011-09-06 Laget: 2011-08-11 Sist oppdatert: 2011-11-14bibliografisk kontrollert

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