Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Enzymes and electron transport in microbial chlorate respiration
Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemi och biomedicinsk vetenskap.
2008 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Microbial chlorate respiration plays an important role in the turnover of oxochlorates in nature and industrial waste management. This thesis deals with the characterization of the molecular components of chlorate respiration in Ideonella dechloratans. Chlorate respiration utilizes two soluble periplasmic enzymes, chlorate reductase and chlorite dismutase, to convert chlorate to chloride and oxygen. The genes encoding the enzymes participating in the chlorate degradation have been sequenced, and are found in close proximity, forming a gene cluster for chlorate metabolism. This work also includes the successful recombinant expression of three genes from Ideonella dechloratans. Two of the gene products, chlorite dismutase and the C subunit of chlorate reductase, participate in the chlorate respiration. The third gene, which is found close to the gene cluster for chlorate metabolism, encodes a soluble c-type cytochrome. The localization of the gene suggests the corresponding protein as a candidate for a role as electron donor to chlorate reductase. Also, the role of soluble periplasmic c cytochromes of Ideonella dechloratans in chlorate respiration was studied. At least one of the soluble c cytochromes was found capable of serving as electron donor for chlorate reduction. This c cytochrome, and several others, can also donate electrons to a terminal oxidase for subsequent reduction of oxygen, as required for the branched electron flow during chlorate respiration.

sted, utgiver, år, opplag, sider
Karlstad: Karlstad University , 2008. , s. 36
Serie
Karlstad University Studies, ISSN 1403-8099 ; 2008:36
Emneord [en]
Ideonella dechloratans, c cytochromes, chlorate, chlorate reductase, chlorite dismutase, heterologous expression, heme reconstitution, electron transport, oxidoreductas
HSV kategori
Forskningsprogram
Kemi
Identifikatorer
URN: urn:nbn:se:kau:diva-2805ISBN: 978-91-7063-196-2 (tryckt)OAI: oai:DiVA.org:kau-2805DiVA, id: diva2:37586
Disputas
2008-10-17, 9C 204, Ericssonsalen, Karlstads universitet, Karlstad, 10:30 (svensk)
Opponent
Veileder
Tilgjengelig fra: 2008-10-13 Laget: 2008-10-08 Sist oppdatert: 2018-01-23bibliografisk kontrollert
Delarbeid
1. Cloning, characterisation, and expression of a novel gene encoding chlorite dismutase from Ideonella dechloratans
Åpne denne publikasjonen i ny fane eller vindu >>Cloning, characterisation, and expression of a novel gene encoding chlorite dismutase from Ideonella dechloratans
2002 (engelsk)Inngår i: Biochimica et Biophysica Acta, Gene Structure and Expression, ISSN 0167-4781, E-ISSN 1879-2634, Vol. 1577, nr 1, s. 445-451Artikkel i tidsskrift (Fagfellevurdert) Published
Emneord
Ideonella dechloratans; Chlorate; Chlorite dismutase; Gene; Expression; Transcription start analysis
HSV kategori
Forskningsprogram
Kemi
Identifikatorer
urn:nbn:se:kau:diva-2797 (URN)10.1016/S0167-4781(02)00446-3 (DOI)
Tilgjengelig fra: 2008-10-07 Laget: 2008-10-07 Sist oppdatert: 2017-12-11bibliografisk kontrollert
2. A Gene Cluster for Chlorate Metabolism in Ideonella dechloratans
Åpne denne publikasjonen i ny fane eller vindu >>A Gene Cluster for Chlorate Metabolism in Ideonella dechloratans
2003 (engelsk)Inngår i: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 69, nr 9, s. 5585-5592Artikkel i tidsskrift (Fagfellevurdert) Published
Forskningsprogram
Kemi
Identifikatorer
urn:nbn:se:kau:diva-2798 (URN)10.1128/AEM.69.9.5585-5592.2003 (DOI)
Tilgjengelig fra: 2008-10-07 Laget: 2008-10-07 Sist oppdatert: 2017-12-11bibliografisk kontrollert
3. The C subunit of Ideonella dechloratans chlorate reductase: Expression, purification, refolding, and heme reconstitution
Åpne denne publikasjonen i ny fane eller vindu >>The C subunit of Ideonella dechloratans chlorate reductase: Expression, purification, refolding, and heme reconstitution
2005 (engelsk)Inngår i: Protein Expression and Purification, ISSN 1046-5928, E-ISSN 1096-0279, Vol. 41, nr 2, s. 306-312Artikkel i tidsskrift (Fagfellevurdert) Published
Emneord
Ideonella dechloratans; Chlorate; Chlorate reductase; ClrC; Heme; Expression; Reconstitution
Identifikatorer
urn:nbn:se:kau:diva-2799 (URN)10.1016/j.pep.2005.02.002 (DOI)
Tilgjengelig fra: 2008-10-07 Laget: 2008-10-07 Sist oppdatert: 2017-12-11bibliografisk kontrollert
4. Expression, refolding and reconstitution of a c-type cytochrome of Ideonella dechloratans
Åpne denne publikasjonen i ny fane eller vindu >>Expression, refolding and reconstitution of a c-type cytochrome of Ideonella dechloratans
Vise andre…
(engelsk)Manuskript (Annet vitenskapelig)
HSV kategori
Forskningsprogram
Kemi
Identifikatorer
urn:nbn:se:kau:diva-2800 (URN)
Tilgjengelig fra: 2008-10-07 Laget: 2008-10-07 Sist oppdatert: 2018-01-23bibliografisk kontrollert
5. Periplasmic c Cytochromes and Chlorate Reduction in Ideonella dechloratans
Åpne denne publikasjonen i ny fane eller vindu >>Periplasmic c Cytochromes and Chlorate Reduction in Ideonella dechloratans
2009 (engelsk)Inngår i: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 75, s. 2439-2445Artikkel i tidsskrift (Fagfellevurdert) Published
HSV kategori
Forskningsprogram
Kemi
Identifikatorer
urn:nbn:se:kau:diva-2804 (URN)10.1128/AEM.01325-08 (DOI)
Merknad

Artikelns titel i Anna Smedja Bäcklunds licentiatuppsats (2009): "Periplasmic c cytochromes coupled to chlorate reduction in Ideonella dechloratans" Artikelns titel i Jan Bohlins doktorsavhandling (2008): "Periplasmic c cytochromes coupled to chlorate reduction in Ideonella dechloratans"

Tilgjengelig fra: 2008-10-08 Laget: 2008-10-08 Sist oppdatert: 2018-01-23bibliografisk kontrollert

Open Access i DiVA

fulltekst(842 kB)1000 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 842 kBChecksum SHA-512
d08b99739cbfb667c426783ce4e50ebf11b5b6243082f86023be003b9b033db0ecbeb8e8eb9da2bfbe25d44064da1bf8f9d3e3dfb5604fa003a877f455c94a0f
Type fulltextMimetype application/pdf

Personposter BETA

Bohlin, Jan

Søk i DiVA

Av forfatter/redaktør
Bohlin, Jan
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 1000 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

isbn
urn-nbn

Altmetric

isbn
urn-nbn
Totalt: 511 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf