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Cloning, characterisation, and expression of a novel gene encoding chlorite dismutase from Ideonella dechloratans
Karlstads universitet, Institutionen för kemi.
Karlstads universitet, Institutionen för kemi.
Karlstads universitet, Institutionen för kemi.
Karlstads universitet, Institutionen för kemi.
2002 (Engelska)Ingår i: Biochimica et Biophysica Acta, Gene Structure and Expression, ISSN 0167-4781, E-ISSN 1879-2634, Vol. 1577, nr 1, s. 445-451Artikel i tidskrift (Refereegranskat) Published
Ort, förlag, år, upplaga, sidor
2002. Vol. 1577, nr 1, s. 445-451
Nyckelord [en]
Ideonella dechloratans; Chlorate; Chlorite dismutase; Gene; Expression; Transcription start analysis
Nationell ämneskategori
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
Forskningsämne
Kemi
Identifikatorer
URN: urn:nbn:se:kau:diva-2797DOI: 10.1016/S0167-4781(02)00446-3OAI: oai:DiVA.org:kau-2797DiVA, id: diva2:37545
Tillgänglig från: 2008-10-07 Skapad: 2008-10-07 Senast uppdaterad: 2017-12-11Bibliografiskt granskad
Ingår i avhandling
1. Enzymes and electron transport in microbial chlorate respiration
Öppna denna publikation i ny flik eller fönster >>Enzymes and electron transport in microbial chlorate respiration
2008 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Microbial chlorate respiration plays an important role in the turnover of oxochlorates in nature and industrial waste management. This thesis deals with the characterization of the molecular components of chlorate respiration in Ideonella dechloratans. Chlorate respiration utilizes two soluble periplasmic enzymes, chlorate reductase and chlorite dismutase, to convert chlorate to chloride and oxygen. The genes encoding the enzymes participating in the chlorate degradation have been sequenced, and are found in close proximity, forming a gene cluster for chlorate metabolism. This work also includes the successful recombinant expression of three genes from Ideonella dechloratans. Two of the gene products, chlorite dismutase and the C subunit of chlorate reductase, participate in the chlorate respiration. The third gene, which is found close to the gene cluster for chlorate metabolism, encodes a soluble c-type cytochrome. The localization of the gene suggests the corresponding protein as a candidate for a role as electron donor to chlorate reductase. Also, the role of soluble periplasmic c cytochromes of Ideonella dechloratans in chlorate respiration was studied. At least one of the soluble c cytochromes was found capable of serving as electron donor for chlorate reduction. This c cytochrome, and several others, can also donate electrons to a terminal oxidase for subsequent reduction of oxygen, as required for the branched electron flow during chlorate respiration.

Ort, förlag, år, upplaga, sidor
Karlstad: Karlstad University, 2008. s. 36
Serie
Karlstad University Studies, ISSN 1403-8099 ; 2008:36
Nyckelord
Ideonella dechloratans, c cytochromes, chlorate, chlorate reductase, chlorite dismutase, heterologous expression, heme reconstitution, electron transport, oxidoreductas
Nationell ämneskategori
Kemi
Forskningsämne
Kemi
Identifikatorer
urn:nbn:se:kau:diva-2805 (URN)978-91-7063-196-2 (ISBN)
Disputation
2008-10-17, 9C 204, Ericssonsalen, Karlstads universitet, Karlstad, 10:30 (Svenska)
Opponent
Handledare
Tillgänglig från: 2008-10-13 Skapad: 2008-10-08 Senast uppdaterad: 2018-01-23Bibliografiskt granskad

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Biochimica et Biophysica Acta, Gene Structure and Expression
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

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