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Electron transport in microbial chlorate respiration
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
2009 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

Several bacterial species are capable to use perchlorate and/or chlorate as an alternative electron acceptor in absence of oxygen. Microbial respiration of oxochlorates is important for biotreatment of effluent from industries where oxochlorates are produced or handled. One of these species, the Gram-negative Ideonella dechloratans, is able to reduce chlorate but not perchlorate. Two soluble enzymes, chlorate reductase and chlorite dismutase, participate in the conversion of chlorate into chloride and molecular oxygen. The present study deals with the electron transport from the membrane-bound components to the periplasmic chlorate reductase. Soluble c cytochromes were investigated for their ability to serve as electron donors to chlorate reductase. The results show that a 6 kDa c cytochrome serves as electron donor for chlorate reductase. This cytochrome also serves as electron donor for a terminal oxidase in the reduction of oxygen that is produced in the course of chlorate respiration. A gene encoding a soluble c cytochrome was found in close proximity to the gene cluster for chlorate reduction. This gene was cloned and expressed heterologously, and the resulting protein was investigated as a candidate electron donor for chlorate reductase. Electron transfer from this protein could not be demonstrated, suggesting that the gene product does not serve as immediate electron donor for chlorate reductase.

 

Place, publisher, year, edition, pages
Karlstad: Karlstad University , 2009. , p. 24
Series
Karlstad University Studies, ISSN 1403-8099 ; 2009:18
Keywords [en]
c cytochromes, chlorate reduction, electron transport
Keywords [sv]
c cytokromer, kloratreduktion, elektrontransport
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-3777ISBN: 978-91-7063-241-9 (print)OAI: oai:DiVA.org:kau-3777DiVA, id: diva2:202275
Presentation
2009-04-17, Ericssonsalen, 9C 204, Karlstads universitet, Karlstad, 13:15 (English)
Opponent
Supervisors
Available from: 2009-03-20 Created: 2009-03-09 Last updated: 2011-11-14Bibliographically approved
List of papers
1. Periplasmic c Cytochromes and Chlorate Reduction in Ideonella dechloratans
Open this publication in new window or tab >>Periplasmic c Cytochromes and Chlorate Reduction in Ideonella dechloratans
2009 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 75, p. 2439-2445Article in journal (Refereed) Published
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-2804 (URN)10.1128/AEM.01325-08 (DOI)
Note

Artikelns titel i Anna Smedja Bäcklunds licentiatuppsats (2009): "Periplasmic c cytochromes coupled to chlorate reduction in Ideonella dechloratans" Artikelns titel i Jan Bohlins doktorsavhandling (2008): "Periplasmic c cytochromes coupled to chlorate reduction in Ideonella dechloratans"

Available from: 2008-10-08 Created: 2008-10-08 Last updated: 2018-01-23Bibliographically approved
2. Characterization of a candidate cytochome c gene associated with the gene cluster for chlorate respiration in Ideonella dechloratans
Open this publication in new window or tab >>Characterization of a candidate cytochome c gene associated with the gene cluster for chlorate respiration in Ideonella dechloratans
Show others...
(English)Manuscript (Other (popular science, discussion, etc.))
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kau:diva-3828 (URN)
Available from: 2009-03-20 Created: 2009-03-20 Last updated: 2018-01-23Bibliographically approved

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Smedja Bäcklund, Anna

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