Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Viscosity contrast effects in analytical scale chromatography: Evidence and impact
Karlstads universitet, Fakulteten för hälsa, natur- och teknikvetenskap (from 2013), Institutionen för ingenjörs- och kemivetenskaper.ORCID-id: 0000-0003-1819-1709
Univ Western Sydney, Sch Sci & Hlth, Australian Ctr Res Separat Sci, North Parramatta, NSW 2751, Australia..
Karlstads universitet, Fakulteten för hälsa, natur- och teknikvetenskap (from 2013), Institutionen för ingenjörs- och kemivetenskaper.
2017 (Engelska)Ingår i: Microchemical journal (Print), ISSN 0026-265X, E-ISSN 1095-9149, Vol. 130, s. 102-107Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The phenomenon known as viscous fingering has been shown to be very detrimental to separation performance in preparative and size exclusion chromatography, and also in multidimensional HPLC However, there are few reports of viscous fingering in analytical scale high performance liquid chromatography (HPLC), despite samples very often being introduced to the analytical-size HPLC columns in a solvent have a substantially different viscosity than the mobile phase. With this study we aim at first hand to investigate if viscous fingering or any similar and related effects to these in preparative levels also take place in analytical scale HPLC and if so, what impact this have on the separation performance. We could show that not only viscous finger does occur in analytical scale columns but also that peak distortions are apparent already at viscosity ratios between eluent and sample solution approaching unity. The latter indicates that a pre-viscous phenomenon is occurring that could be more important than the viscous fingering itself at the analytical format. As the viscosity contrast increases, the leading edge of the sample band distorts and the band volume increases, both leading to a decrease in performance.

Ort, förlag, år, upplaga, sidor
Elsevier, 2017. Vol. 130, s. 102-107
Nyckelord [en]
Analytical scale HPLC, Viscous fingering, Viscosity contrast, Column efficiency
Nationell ämneskategori
Kemi
Forskningsämne
Kemi
Identifikatorer
URN: urn:nbn:se:kau:diva-62621DOI: 10.1016/j.microc.2016.08.007ISI: 000390072400015OAI: oai:DiVA.org:kau-62621DiVA, id: diva2:1130706
Tillgänglig från: 2017-08-10 Skapad: 2017-08-10 Senast uppdaterad: 2017-08-10Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltext

Sök vidare i DiVA

Av författaren/redaktören
Samuelsson, JörgenFornstedt, Torgny
Av organisationen
Institutionen för ingenjörs- och kemivetenskaper
I samma tidskrift
Microchemical journal (Print)
Kemi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 5 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf