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Use of Dimethylacrylamide Coated Capillaries for the Study of beta2-Glycoprotein I with Affinity Capillary Electrophoresis
Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemi och biomedicinsk vetenskap.
Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemi och biomedicinsk vetenskap.
2003 (engelsk)Inngår i: / [ed] Lars G. Blomberg, Niels H. H. Heegaard, 2003Konferansepaper, Publicerat paper (Annet (populærvitenskap, debatt, mm))
Abstract [en]

The purpose of this work is to develop a solution method for examining interactions of human beta2-Glycoprotein I (b2gpI) with antibodies and natural ligands under nondenaturing conditions. In the approach, a physiological pH was applied because our intention was to study the interactions between b2gpI and antibodies and the protein must thus be kept in its native state. Preliminary experiments have shown that the recovery of the protein, when analyzed in an uncoated capillary, is poor. At physiological pH the protein is positively charged and most likely it was adsorbed at the negatively charged capillary wall in these experiments. Therefore dimethylacrylamide (DMA) coated capillaries1 are used and analyses could be performed with acceptable recovery. Interaction studies are performed simply by adding the antibody or ligand to the running buffer. The method showed good repeatability and efficiency

sted, utgiver, år, opplag, sider
2003.
HSV kategori
Forskningsprogram
Kemi
Identifikatorer
URN: urn:nbn:se:kau:diva-25266OAI: oai:DiVA.org:kau-25266DiVA, id: diva2:599042
Konferanse
Analysdagarna 2003, Göteborg
Tilgjengelig fra: 2013-01-22 Laget: 2013-01-22 Sist oppdatert: 2013-01-22

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