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Olsson, Jeanette
Publications (5 of 5) Show all publications
Olsson, J. & Blomberg, L. G. (2008). Enantioseparation of Omeprazole and its Metabolite 5-Hydroxyomeprazole using Open Tubular Capillary Electrochromatography with Immobilized Avidin as Chiral Selector. Journal of chromatography. B, 875(1), 329-332
Open this publication in new window or tab >>Enantioseparation of Omeprazole and its Metabolite 5-Hydroxyomeprazole using Open Tubular Capillary Electrochromatography with Immobilized Avidin as Chiral Selector
2008 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 875, no 1, p. 329-332Article in journal (Refereed) Published
Abstract [en]

The present paper demonstrates the enantiomeric separation of omeprazole and its metabolite 5-hydroxyomeprazole performed with open tubular capillary electrochromatography (OT-CEC). The protein avidin was used as the chiral selector. Avidin was immobilized by a Schiffs base type of reaction where the protein was via glutaraldehyde covalently bonded to the amino-modified wall of a fused-silica capillary, 50 μm i.d. Both racemates were baseline resolved. Resolution was 1.9 and 2.3, respectively, using ammonium acetate buffer, pH 5.8, 5% methanol, with UV-detection. These values of resolution using OT-CEC are higher than earlier published results regarding chiral separation of omeprazole and 5-hydroxyomeprazole on packed CEC. The number of theoretical plates also indicated good separation efficiency.

National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-2028 (URN)10.1016/j.jchromb.2008.05.050 (DOI)
Available from: 2008-04-17 Created: 2008-04-17 Last updated: 2017-12-07Bibliographically approved
Olsson, J. (2008). New Techniques for Chiral Separations. (Doctoral dissertation). Karlstad: Karlstads universitet
Open this publication in new window or tab >>New Techniques for Chiral Separations
2008 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

Gas chromatography (GC) has been utilized for the study of enantiomer resolution of the atropisomers of PCBs, o,p´-DDD and o,p´-DDT. Different substituents and concentrations of cyclodextrin, capillary dimensions and type of stationary phase films have been investigated to achieve the resolution of as many of the atropisomers on one column as possible. The results indicated that the butyl substitution of 6-hydroxyl and the methyl substitution of 2- and 3-hydroxyl were the most promising for the enantiomeric separation. Using Capillary Electrophoresis (CE), the trimers and monomers of PM-β-CDs were compared for enantiomeric resolution, as well as comparing the cationic PMMA-β-CD with the anionic HS-β-CD. In these studies the trimer did not show an improved resolution for mepivacaine, when compared to the equimolar concentration of the monomer. The cationic CD gave increased resolution values for ibuprofen when compared to the anionic CD. A scheme for reversing enantiomeric elution order of both the basic propranolol and acidic ibuprofen is also presented, with the aim of facilitating the detection of impurities in a high sample loading. The detection of 1% of each enantiomer of propranolol, and 1% of R(-)-ibuprofen, was demonstrated, with elution prior to the tailing peak of the corresponding enantiomer. Dimethylacrylamide-coated capillaries were used in this work, and the stability of this coating was demonstrated, giving a highly reduced electroosmotic flow for up to six months. Enantiomeric baseline separations of omeprazole and 5-hydroxyomeprazole have also been achieved with both CE and Open Tubular Capillary Electrochromatography (OT-CEC) methods. With CE-UV, both a non-aqueous method (using HDMS-β-CD) and an aqueous method (using HS-β-CD) were used for enantiomeric resolution of the two racemates. Resolution of omeprazole was also achieved using CE-Electrospray Ionization-Mass Spectrometry (ESI-MS). In OT-CEC, avidin was immobilized on the inside surface of a fused silica capillary and was employed as chiral selector for the enantiomeric baseline resolution of omeprazole and 5-hydroxyomeprazole.

Place, publisher, year, edition, pages
Karlstad: Karlstads universitet, 2008. p. 60
Series
Karlstad University Studies, ISSN 1403-8099 ; 2008:11
Keywords
analytical chemistry, chiral separation, GC, CE, CEC
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-1594 (URN)978-91-7063-169-6 (ISBN)
Public defence
2008-04-30, Ericssonsalen, 9C 204, Karlstads universitet, Karlstad, 10:15
Opponent
Supervisors
Available from: 2008-04-17 Created: 2008-04-17 Last updated: 2011-12-19
Magnusson, J., Wan, H., Blomberg, L. G. & Olsson, J. (2002). A simple and versatile scheme for reversing enantiomeric elution order and facilitating enantiomeric impurity determination in capillary electrophoresis. Electrophoresis, 23 (2002) 3013-3019
Open this publication in new window or tab >>A simple and versatile scheme for reversing enantiomeric elution order and facilitating enantiomeric impurity determination in capillary electrophoresis
2002 (English)In: Electrophoresis, 23 (2002) 3013-3019Article in journal (Refereed)
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-16640 (URN)
Available from: 2013-01-21 Created: 2013-01-21 Last updated: 2013-01-21
Olsson, J. (2002). Evaluation of modified cyclodextrins for chiral separations in gas chromatography and capillary electrophoresis. (Licentiate dissertation). Karlstad: Karlstad University Studies
Open this publication in new window or tab >>Evaluation of modified cyclodextrins for chiral separations in gas chromatography and capillary electrophoresis
2002 (English)Licentiate thesis, monograph (Other academic)
Abstract [en]

ABSTRACT

An evaluation of different derivatized cyclodextrins as chiral selectors for enantiomer separations has been performed with capillary electrophoresis and gas chromatography. Using gas chromatography, resolution of the enantiomers of atropisomeric PCB congeners, o,p-DDT and o,p-DDD was attempted on columns containing a mix of chiral selectors. Examinations of closely related cyclodextrins and the effect of different concentrations of these chiral selectors were executed.

A method for the determination of enantiomeric trace impurities by capillary electrophoresis was developed. This method consists of a simple and versatile scheme for reversing enantiomeric elution order. Enantiomer purity determination is most often done under overload conditions which lead to deformed peaks. When quantification of the impurity is performed the peaks ought to be fully resolved which can not be done if the minor peak appears on the tailing of a major peak. This can be avoided if the elution order of the enantiomers can be decided. A basic analyte, propranolol, and an acidic analyte, ibuprofen, were used as model substances.

Place, publisher, year, edition, pages
Karlstad: Karlstad University Studies, 2002
Identifiers
urn:nbn:se:kau:diva-19056 (URN)918942297x (ISBN)
Available from: 2013-01-21 Created: 2013-01-21 Last updated: 2013-01-21
Magnusson, J., Blomberg, L. G., Claude, S., Tabacchi, R., Saxer, A., Schürch, S. & Olsson, J. (2000). Gas chromatographic enantiomer separation of atropisomeric PCBs using modified cyclodextrins as chiral phases,. J. High Resolut. Chromatogr., 23 (2000) 619-627
Open this publication in new window or tab >>Gas chromatographic enantiomer separation of atropisomeric PCBs using modified cyclodextrins as chiral phases,
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2000 (Swedish)In: J. High Resolut. Chromatogr., 23 (2000) 619-627Article in journal (Refereed)
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-19711 (URN)
Available from: 2013-01-21 Created: 2013-01-21 Last updated: 2013-01-21
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