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Altun, Zeki
Publications (10 of 12) Show all publications
Blomberg, L. G., Altun, Z., Skoglund, C. & Abdel-Rehim, M. (2010). Monolithic methacrylate packed 96-tips for high throughput bioanalysis. Journal of Chromatography A, 1217(16), 2581-2588
Open this publication in new window or tab >>Monolithic methacrylate packed 96-tips for high throughput bioanalysis
2010 (English)In: Journal of Chromatography A, ISSN 0021-9673, Vol. 1217, no 16, p. 2581-2588Article in journal (Refereed) Published
Place, publisher, year, edition, pages
Elsevier, 2010
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-9885 (URN)10.1016/j.chroma.2009.10.072 (DOI)
Available from: 2012-02-08 Created: 2012-02-08 Last updated: 2015-06-24Bibliographically approved
Altun, Z. (2008). New Techniques for Sample Preparation in Analytical Chemistry: - Microextraction in Packed Syringe (MEPS) and Methacrylate Based Monolithic Pipette Tips. (Doctoral dissertation).
Open this publication in new window or tab >>New Techniques for Sample Preparation in Analytical Chemistry: - Microextraction in Packed Syringe (MEPS) and Methacrylate Based Monolithic Pipette Tips
2008 (English)Doctoral thesis, monograph (Other academic)
Keywords
Liquid Chromatography/Tandem Mass Spectrometry, Local Anaesthetics, Microextraction in Packed Syringe, Monoliths, Pipette Tips, Sample Preparation
Identifiers
urn:nbn:se:kau:diva-21799 (URN)9789170631610 (ISBN)
Available from: 2013-01-21 Created: 2013-01-21 Last updated: 2013-01-21
Altun, Z. (2008). New Techniques for Sample Preparation in Analytical Chemistry: Microextraction in Packed Syringe (MEPS) and Methacrylate Based Monolithic Pipette Tips. (Doctoral dissertation). Fakulteten för teknik- och naturvetenskap
Open this publication in new window or tab >>New Techniques for Sample Preparation in Analytical Chemistry: Microextraction in Packed Syringe (MEPS) and Methacrylate Based Monolithic Pipette Tips
2008 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

Sample preparation is often a bottleneck in systems for chemical analysis. The aim of this work was to investigate and develop new techniques to address some of the shortcomings of current sample preparation methods. The goal has been to provide full automation, on-line coupling to detection systems, short sample preparation times and high-throughput.

In this work a new technique for sample preparation that can be connected on-line to liquid chromatography (LC) and gas chromatography (GC) has been developed. Microextraction in packed syringe (MEPS) is a new solid-phase extraction (SPE) technique that is miniaturized and can be fully automated. In MEPS approximately 1 mg of sorbent material is inserted into a gas tight syringe (100-250 μL) as a plug. Sample preparation takes place on the packed bed. Evaluation of the technique was done by the determination of local anaesthetics in human plasma samples using MEPS on-line with LC and tandem mass spectrometry (MS-MS). MEPS connected to an autosampler was fully automated and clean-up of the samples took about one minute. In addition, in the case of plasma samples the same plug of sorbent could be used for about 100 extractions before it was discarded.

A further aim of this work was to increase sample preparation throughput. To do that disposable pipette tips were packed with a plug of porous polymer monoliths as sample adsorbent and were then used in connection with 96-well plates and LC-MS-MS. The evaluation of the methods was done by the analysis of local anaesthetics lidocaine and ropivacaine, and anti-cancer drug roscovitine in plasma samples. When roscovitine and lidocaine in human plasma and water samples were used as model substances, a 96-plate was handled in about two minutes. Further, disposable pipette tips may be produced at low cost and because they are used only once, carry-over is eliminated.

Place, publisher, year, edition, pages
Fakulteten för teknik- och naturvetenskap, 2008. p. 66
Series
Karlstad University Studies, ISSN 1403-8099 ; 2008:4
Keywords
Liquid Chromatography/Tandem Mass Spectrometry, Local Anaesthetics, Microextraction in Packed Syringe, Monoliths, Pipette Tips, Sample Preparation
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-1420 (URN)978-91-7063-161-0 (ISBN)
Public defence
2008-02-22, 9C204, Universitetsgatan 2, 651 88, Karlstad, 10:15
Opponent
Supervisors
Available from: 2008-02-12 Created: 2008-02-12 Last updated: 2011-11-09
Altun, Z., Jagerdeo, E., Blomberg, L. G. & Abdel-Rehim, M. (2006). Drug screening using microextraction in packed syringe (MEPS) / LC-MS utilizing monolithic-based sorbent material,. Journal of Liquid Chromatography and Related Technologies, 29 (2006) 829-839
Open this publication in new window or tab >>Drug screening using microextraction in packed syringe (MEPS) / LC-MS utilizing monolithic-based sorbent material,
2006 (English)In: Journal of Liquid Chromatography and Related Technologies, 29 (2006) 829-839Article in journal (Refereed)
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-18459 (URN)
Available from: 2013-01-21 Created: 2013-01-21 Last updated: 2013-01-21
Blomberg, L. G., Altun, Z., Altun, Z. & Abdel-Rehim, M. (2006). Increasing Sample Preparation Throughput Using Monolithic Methacrylate Polymer as Packing Material for 96-Tips. J. Chromatogr. B, J. Liq. Chromatogr. & Rel. Technol., 29 (2006) 1725-1736
Open this publication in new window or tab >>Increasing Sample Preparation Throughput Using Monolithic Methacrylate Polymer as Packing Material for 96-Tips
2006 (English)In: J. Chromatogr. B, J. Liq. Chromatogr. & Rel. Technol., 29 (2006) 1725-1736Article in journal (Refereed)
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-20279 (URN)
Available from: 2013-01-21 Created: 2013-01-21 Last updated: 2013-01-21
Abdel-Rehim, M., Andersson, L., Altun, Z. & Blomberg, L. G. (2006). Microextraction in Packed Syringe Online with Liquid Chromatography-Tandem Mass Spectrometry: Molecularly imprinted polymer as packing material for MEPS in selective extraction of ropivacaine from plasma. Journal of Liquid Chromatography & Related Technologies, 29(12), 1725-1736
Open this publication in new window or tab >>Microextraction in Packed Syringe Online with Liquid Chromatography-Tandem Mass Spectrometry: Molecularly imprinted polymer as packing material for MEPS in selective extraction of ropivacaine from plasma
2006 (English)In: Journal of Liquid Chromatography & Related Technologies, ISSN 1082-6076, E-ISSN 1520-572X, Vol. 29, no 12, p. 1725-1736Article in journal (Refereed) Published
Abstract [en]

The excellent performance of a new sample preparation method, microextraction in packed syringe (MEPS), was recently illustrated by online LC‐MS and GS‐MS assays of local anaesthetics in plasma samples. In the method, approximately 1 mg of solid packing material was inserted into a syringe (100–250 µL) as a plug. Sample preparation took place on the packed bed. The new method was easy to use, fully automated, of low cost, and rapid in comparison with previously used methods. This paper presents the use of molecularly imprinted polymers (MIPs) as packing material for higher extraction selectivity. Development and validation of a method for MIP‐MEPS online with LC‐MS‐MS using ropivacaine in plasma as model compound were investigated. A bupivacaine imprinted polymer was used. The method was validated and the standard curves were evaluated by means of quadratic regression and weighted by inverse of the concentration: 1/x for the calibration range 2–2000 nM. The applied polymer could be used more than 100 times before the syringe was discarded. The extraction recovery was 60%. The results showed high correlation coefficients (R 2 >0.999) for all runs. The accuracy, given as a percentage deviation from the nominal concentration values, ranged from -6% to 3%. The precision, given as the relative standard deviation, at three different concentrations (QC samples) was consistently about 3% to 10%. The limit of quantification was 2 nM.

Keywords
Column liquid chromatography‐tandem mass spectrometry, Column liquid chromatography-tandem mass spectrometry, Molecularly imprinted polymers, Ropivacaine, Sample preparation
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-21457 (URN)10.1080/10826070600716843 (DOI)
Available from: 2013-01-21 Created: 2013-01-21 Last updated: 2017-12-06Bibliographically approved
Altun, Z., Blomberg, L. G. & Abdel-Rehim, M. (2005). Increasing Sample Preparation Throughput Using Monolithic Methacrylate Polymer as Packing Material for 96-Tips: 2 Minutes per 96-Well Plate.
Open this publication in new window or tab >>Increasing Sample Preparation Throughput Using Monolithic Methacrylate Polymer as Packing Material for 96-Tips: 2 Minutes per 96-Well Plate
2005 (English)Manuscript (preprint) (Other academic)
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kau:diva-1973 (URN)
Available from: 2008-02-05 Created: 2008-02-05 Last updated: 2011-11-09Bibliographically approved
Blomberg, L. G. & Altun, Z. (2005). New Techniques for Sample Preparation in Analytical Chemistry. (Licentiate dissertation).
Open this publication in new window or tab >>New Techniques for Sample Preparation in Analytical Chemistry
2005 (English)Licentiate thesis, monograph (Other academic)
Series
Karlstad University Press, 2005: 24
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-21797 (URN)
Available from: 2013-01-21 Created: 2013-01-21 Last updated: 2013-01-21
Abdel-Rehim, M., Altun, Z. & Blomberg, L. G. (2004). New trend in sample preparation: On-line microextraction in packed syringe (MEPS) for LC and GC applications, Part II, Determination of ropivacaine and its metabolites in human plasma samples using MEPS-LC-MS-MS. J. Mass Spectr., 39 (2004) 1488-1493
Open this publication in new window or tab >>New trend in sample preparation: On-line microextraction in packed syringe (MEPS) for LC and GC applications, Part II, Determination of ropivacaine and its metabolites in human plasma samples using MEPS-LC-MS-MS
2004 (English)In: J. Mass Spectr., 39 (2004) 1488-1493Article in journal (Refereed)
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-21801 (URN)
Available from: 2013-01-21 Created: 2013-01-21 Last updated: 2013-01-21
Altun, Z., Abdel-Rehim, M. & Blomberg, L. G. (2004). New trends in sample preparation: On-line microextraction in packed syringe (MEPS) for LC and GC applications. Part III Determination and validation of local anaesthetics in human plasma samples using a cation-exchange sorbent and MEPS-LC-MS-MS. J. Chromatogr. B, 813 (2004) 129-135
Open this publication in new window or tab >>New trends in sample preparation: On-line microextraction in packed syringe (MEPS) for LC and GC applications. Part III Determination and validation of local anaesthetics in human plasma samples using a cation-exchange sorbent and MEPS-LC-MS-MS
2004 (English)In: J. Chromatogr. B, 813 (2004) 129-135Article in journal (Refereed)
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-21802 (URN)
Available from: 2013-01-21 Created: 2013-01-21 Last updated: 2013-01-21
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