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Nånberg, Eewa
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Publications (10 of 48) Show all publications
Shu, H., Jönsson, B., Gennings, C., Lindh, C., Nånberg, E. & Bornehag, C.-G. (2019). PVC flooring at home and uptake of phthalates in pregnant women. Indoor Air (1), 43-54
Open this publication in new window or tab >>PVC flooring at home and uptake of phthalates in pregnant women
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2019 (English)In: Indoor Air, ISSN 0905-6947, E-ISSN 1600-0668, no 1, p. 43-54Article in journal (Other academic) Published
Abstract [en]

Phthalates are used as plasticizers in polyvinyl chloride (PVC) materials and it is known that phthalates may migrate into the surrounding environment and then become a source for human uptake. The aim of the study was to investigate whether residential PVC flooring was related to the urinary levels of phthalate metabolites determined in pregnant women. The data were from the Swedish SELMA study where sampling was conducted during the time period 2007-2010. Spot urine samples from 1674 women at the end of the first trimester were analyzed for 14 metabolites from seven phthalates and one phthalate alternative. Data on flooring material in the kitchen and the parents' bedrooms as well as potential confounders were collected by postal questionnaires at the same time as the urine samples were taken. Multiple regression modeling by least square geometric mean and weighted quantile sum regression was applied to log-transformed and creatinine-adjusted phthalate metabolite concentrations adjusted for potential confounders from questionnaire data. This study has found significantly higher urinary levels of the BBzP metabolite (MBzP) in pregnant women living in homes with PVC flooring as compared to homes with other flooring materials

Place, publisher, year, edition, pages
Wiley-Blackwell, 2019
Keywords
Human Breast-Milk; Anogenital Distance; Urinary Concentrations; Prenatal Exposure; Care Products; Male Infants; Metabolites; Association; Dust; Bisphenols
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Identifiers
urn:nbn:se:kau:diva-62630 (URN)10.1111/ina.12508 (DOI)
Available from: 2017-08-11 Created: 2017-08-11 Last updated: 2019-03-14Bibliographically approved
Shu, H., Wikstrom, S., Jonsson, B. A. G., Lindh, C. H., Svensson, Å., Nånberg, E. & Bornehag, C.-G. (2018). Prenatal phthalate exposure was associated with croup in Swedish infants. Acta Paediatrica, 107(6), 1011-1019
Open this publication in new window or tab >>Prenatal phthalate exposure was associated with croup in Swedish infants
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2018 (English)In: Acta Paediatrica, ISSN 0803-5253, E-ISSN 1651-2227, Vol. 107, no 6, p. 1011-1019Article in journal (Refereed) Published
Abstract [en]

Aim: This study examined whether prenatal phthalate exposure was associated with lower or upper airway inflammation in infants. Methods: From 2007 to 2010, we used liquid chromatography-tandem mass spectrometry, adjusted for creatinine, to analyse 14 phthalate metabolites and one phthalate replacement in the urine of 1062 Swedish mothers at a median of 10 weeks of pregnancy. This was used to determine any associations between prenatal phthalate exposure and croup, wheezing or otitis in their offspring until 12 months of age, using logistic regression, adjusted for potential confounders. Results: There were significant associations between phthalate metabolites of butyl-benzyl phthalate (BBzP) and di-ethyl-hexyl phthalate (DEHP) concentrations in maternal prenatal urine and croup in 1062 infants during the first year of life, when adjusted for potential confounders. A dose-response relationship was found between prenatal phthalates exposure and maternal reported croup in the children, with a significant association in boys. There was no clear indication with regard to associations between prenatal phthalate exposure and wheezing or otitis media in the children during the first year of life. Conclusion: Our analysis suggests that exposure to BBzP and DEHP phthalates was associated with maternal reports of croup in infants up to 12 months of age

Place, publisher, year, edition, pages
John Wiley & Sons, 2018
National Category
Pediatrics Public Health, Global Health, Social Medicine and Epidemiology Respiratory Medicine and Allergy
Identifiers
urn:nbn:se:kau:diva-67368 (URN)10.1111/apa.14245 (DOI)000431956700017 ()29385277 (PubMedID)
Available from: 2018-05-24 Created: 2018-05-24 Last updated: 2018-07-10Bibliographically approved
Shu, H., Jönsson, B. A., Gennings, C., Svensson, Å., Nånberg, E., Lindh, C. H., . . . Bornehag, C.-G. (2018). Temporal Trends of Phthalate Exposures during 2007-2010 in Swedish Pregnant Women. Journal of Exposure Science and Environmental Epidemiology, 28(5), 437-447
Open this publication in new window or tab >>Temporal Trends of Phthalate Exposures during 2007-2010 in Swedish Pregnant Women
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2018 (English)In: Journal of Exposure Science and Environmental Epidemiology, ISSN 1559-0631, E-ISSN 1559-064X, Vol. 28, no 5, p. 437-447Article in journal (Refereed) Published
Abstract [en]

Background: The general population is exposed to phthalates, a group of chemicals with strong evidence for endocrine disrupting properties, commonly used in a large number of consumer products. Based on published research and evidence compiled by environmental agencies, certain phthalate applications and products have become restricted, leading to an increasing number of “new generation compounds” coming onto the market during recent years replacing older phthalates. Some examples of such newer compounds are di-iso-nonyl phthalate (DiNP), di-iso-decyl phthalate (DiDP), and most recently di-isononyl-cyclohexane-1,2-dicarboxylate (DiNCH). Objectives: In order to evaluate temporal trends in phthalate exposure, first trimester urinary biomarkers of phthalates were measured in the Swedish SELMA study over a period of 2.5 years (2007–2010). Methods: We collected first morning void urine samples around week 10 of pregnancy from 1651 pregnant women. Spot samples were analyzed for 13 phthalate metabolites and one phthalate replacement and least square geometric mean (LSGM) levels of the metabolites were compared between the sampling years when adjusted for potential confounders. Results: All 14 metabolites were detectable in more than 99% of the SELMA subjects. The levels were generally comparable to other studies, but the SELMA subjects showed slightly higher exposure to butyl-benzyl phthalate (BBzP) and di-butyl phthalate (DBP). Di-ethyl-hexyl phthalate (DEHP) metabolites levels decreased while DiNP, DiDP/di-2-propylheptyl phthalate (DPHP), and DiNCH metabolites levels increased during the sampling period. Conclusions: Urinary metabolite levels of the older phthalates and more recently introduced phthalate replacement compound changed during the short sampling period in this Swedish pregnancy cohort. Our results indicate that replacement of phthalates can make an impact on human exposure to these chemicals. During this particularly vulnerable stage of life, phthalate exposures are of particular concern as the impacts, though not immediately noticeable, may increase the risk for health effects later in life.

Place, publisher, year, edition, pages
Nature Publishing Group, 2018
Keywords
DiNCH, Endocrine disrupting chemicals, Exposure, Phthalates, Pregnant, SELMA-Study, Temporal
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Research subject
Public Health Science
Identifiers
urn:nbn:se:kau:diva-62628 (URN)10.1038/s41370-018-0020-6 (DOI)000444446100003 ()2-s2.0-85042354826 (Scopus ID)
Available from: 2017-08-11 Created: 2017-08-11 Last updated: 2019-06-17Bibliographically approved
Rendel, F., Fjaeraa Alfredsson, C., Bornehag, C.-G., Sundström, B. E. & Nånberg, E. (2017). Effects of Di-Isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells. Basic & Clinical Pharmacology & Toxicology, 120(3), 218-323
Open this publication in new window or tab >>Effects of Di-Isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells
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2017 (English)In: Basic & Clinical Pharmacology & Toxicology, ISSN 1742-7835, E-ISSN 1742-7843, Vol. 120, no 3, p. 218-323Article in journal (Refereed) Published
Place, publisher, year, edition, pages
Wiley-Blackwell, 2017
Keywords
phthlate, DiNP, MiNP, NPY, SH-SY5Y neuroblastoma cells
National Category
Medical and Health Sciences Cell and Molecular Biology
Research subject
Biomedical Sciences; Biomedical Sciences
Identifiers
urn:nbn:se:kau:diva-45720 (URN)10.1111/bcpt.12670 (DOI)000394538600016 ()27625336 (PubMedID)
Available from: 2016-09-05 Created: 2016-09-05 Last updated: 2019-06-10Bibliographically approved
Fjæraa Alfredsson, C., Rendel, F., Liang, Q.-L., Sundström, B. E. & Nånberg, E. (2015). Altered sensitivity to ellagic acid in neuroblastoma cells undergoing differentiation with 12-0-tetradecanoylphorbol-13-acetate and all-trans retinoic acid. Biomedicine and Pharmacotherapy, 76, 39-45
Open this publication in new window or tab >>Altered sensitivity to ellagic acid in neuroblastoma cells undergoing differentiation with 12-0-tetradecanoylphorbol-13-acetate and all-trans retinoic acid
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2015 (English)In: Biomedicine and Pharmacotherapy, ISSN 0753-3322, E-ISSN 1950-6007, Vol. 76, p. 39-45Article in journal (Refereed) Published
Abstract [en]

Ellagic acid has previously been reported to induce reduced proliferation and activation of apoptosis in several tumor cell lines including our own previous data from non-differentiated human neuroblastoma SH-SY5Y cells. The aim of this study was now to investigate if in vitro differentiation with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate or the vitamin A derivative all-trans retinoic acid altered the sensitivity to ellagic acid in SH-SY5Y cells. The methods used were cell counting and LDH-assay for evaluation of cell number and cell death, flow cytometric analysis of SubG(1)-and TUNEL-analysis for apoptosis and western blot for expression of apoptosis-associated proteins. In vitro differentiation was shown to reduce the sensitivity to ellagic acid with respect to cell detachment, loss of viability and activation of apoptosis. The protective effect was phenotype-specific and most prominent in all-trans retinoic acid-differentiated cultures. Differentiation-dependent up-regulation of Bcl-2 and integrin expression is introduced as possible protective mechanisms. The presented data also point to a positive correlation between proliferative activity and sensitivity to ellagic-acid-induced cell detachment. In conclusion, the presented data emphasize the need to consider degree of neuronal differentiation and phenotype of neuroblastoma cells when discussing a potential pharmaceutical application of ellagic acid in tumor treatment.

Keywords
Ellagic acid, Cell adhesion, Apoptosis, Neuroblastoma, Differentiation
National Category
Basic Medicine
Research subject
Biomedical Sciences
Identifiers
urn:nbn:se:kau:diva-29903 (URN)10.1016/j.biopha.2015.10.008 (DOI)000367541500007 ()
Available from: 2013-10-24 Created: 2013-10-24 Last updated: 2019-07-09Bibliographically approved
Ma, P., Liu, X., Wu, J., Yan, B., Zhang, Y., Lu, Y., . . . Yang, X. (2015). Cognitive deficits and anxiety induced by diisononyl phthalate in mice and the neuroprotective effects of melatonin. Scientific Reports, 5, Article ID 14676.
Open this publication in new window or tab >>Cognitive deficits and anxiety induced by diisononyl phthalate in mice and the neuroprotective effects of melatonin
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2015 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 5, article id 14676Article in journal (Refereed) Published
Abstract [en]

Diisononyl phthalate (DINP) is a plasticizer that is frequently used as a substitute for other plasticizers whose use is prohibited in certain products. In vivo studies on the neurotoxicity of DINP are however, limited. This work aims to investigate whether DINP causes neurobehavioral changes in mice and to provide useful advice on preventing the occurrence of these adverse effects. Behavioral analysis showed that oral administration of 20 or 200â mg/kg/day DINP led to mouse cognitive deficits and anxiety. Brain histopathological observations, immunohistochemistry assays (cysteine-aspartic acid protease 3 [caspase-3], glial fibrillary acidic protein [GFAP]), oxidative stress assessments (reactive oxygen species [ROS], glutathione [GSH], superoxide dismutase [SOD] activities, 8-hydroxy-2-deoxyguanosine [8-OH-dG] and DNA-protein crosslinks [DPC]), and assessment of inflammation (tumor necrosis factor alpha [TNF-Crossed D sign°[ and interleukin-1 beta [IL-1β]) of mouse brains showed that there were histopathological alterations in the brain and increased levels of oxidative stress, and inflammation for these same groups. However, some of these effects were blocked by administration of melatonin (50â mg/kg/day). Down-regulation of oxidative stress was proposed to explain the neuroprotective effects of melatonin. The data suggests that DINP could cause cognitive deficits and anxiety in mice, and that melatonin could be used to avoid these adverse effects.

Place, publisher, year, edition, pages
Nature Publishing Group, 2015
Keywords
Immunohistochemistry, Risk factors
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Research subject
Public Health Science
Identifiers
urn:nbn:se:kau:diva-42349 (URN)10.1038/srep14676 (DOI)000362087500001 ()2-s2.0-84942938338 (Scopus ID)
Available from: 2016-06-07 Created: 2016-05-23 Last updated: 2017-12-06Bibliographically approved
Tang, J., Yuan, Y., Wei, C., Liao, X., Yuan, J., Nånberg, E., . . . Yang, X. (2015). Neurobehavioral changes induced by di(2-ethylhexyl) phthalate and the protective effects of vitamin E in Kunming mice. Toxicology research, 4(4), 1006-1015
Open this publication in new window or tab >>Neurobehavioral changes induced by di(2-ethylhexyl) phthalate and the protective effects of vitamin E in Kunming mice
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2015 (English)In: Toxicology research, ISSN 2045-452X, Vol. 4, no 4, p. 1006-1015Article in journal (Refereed) Published
Abstract [en]

Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer commonly used in PVC that may leach into the environment, and has been shown to adversely affect the health of humans and animals. We undertook a study to ascertain the neurotoxicity of DEHP in Kunming mice. This study included three rounds of testing. In the first round, Kunming mice were exposed to different concentrations of DEHP (0, 5, 50, 500 mg kg(-1) per day) after which their cognitive ability was assessed using the Morris water maze (MWM) test. The reactive oxygen species (ROS) content in tissue and the malondialdehyde (MDA) content of brains were also measured. In the second round, vitamin E (50 mg kg(-1) per day) was given daily as an anti-oxidant via the intragastric route. Cognitive deficits and locomotor activity, as well as ROS and MDA contents were tested employing the same methods. In the third round, the depressive mood of mice after DEHP exposure (500 mg kg(-1) per day) was measured using the open field test, the tail suspension test, and the forced swim test. The main findings of this study include: (1) a statistical association exists between DEHP oral exposure and spatial learning (DEHP 500 mg kg(-1) per day) and memory (DEHP 50 mg kg(-1) per day) dysfunction as ascertained by an MWM test of Kunming mice. (2) A statistical association was also found between DEHP oral exposure (50 and 500 mg kg(-1) per day) and oxidative stress (ROS and MDA) of mouse brain tissue. (3) Co-administration of vitamin E (50 mg kg(-1) per day) diminishes the elevation of ROS and MDA induced by DEHP (50 mg kg(-1) per day) from significant levels to non-significant levels. (4) Co-administration of vitamin E (50 mg kg(-1) per day) protects against mouse memory dysfunction induced by DEHP (50 mg kg(-1) per day) from being significant to being not significant. (5) In the 5 mg kg(-1) per day DEHP exposure groups, oxidative stress in brain tissue, and neurobehavioral changes were not found. (6) High dose DEHP exposure (500 mg kg(-1) per day) may induce behavioral despair in mice. Conclusions: These data suggest that DEHP is neurotoxic with regard to cognitive ability and locomotor activity.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2015
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Research subject
Public Health Science
Identifiers
urn:nbn:se:kau:diva-41647 (URN)10.1039/c4tx00250d (DOI)000356612300023 ()
Available from: 2016-04-11 Created: 2016-04-11 Last updated: 2016-05-12Bibliographically approved
Fjæraa Alfredsson, C., Ding, M., Liang, Q.-L., Sundström, B. & Nånberg, E. (2014). Ellagic acid induces a dose- and time-dependent depolarization of mitochondria and activation of caspase-9 and -3 in human neuroblastoma cells. Biomedicine and Pharmacotherapy, 68(1), 129-135
Open this publication in new window or tab >>Ellagic acid induces a dose- and time-dependent depolarization of mitochondria and activation of caspase-9 and -3 in human neuroblastoma cells
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2014 (English)In: Biomedicine and Pharmacotherapy, ISSN 0753-3322, E-ISSN 1950-6007, Vol. 68, no 1, p. 129-135Article in journal (Refereed) Published
Abstract [en]

The polyphenol ellagic acid is found in many natural food sources and has been proposed as a candidate compound for clinical applications due to its anti-oxidative capacity and as a potential anti-tumorigenic compound. The objective of the present study was to evaluate the sensitivity to and possible apoptosis mechanism induced by ellagic acid in neuronal tumor cells. As a model the human neuroblastoma SH-SY5Y cell line was used. The methods applied were bright field and phase contrast microscopy, XTT- and LDH-assays, western blot, and flow cytometric analysis of DNA degradation and mitochondrial membrane potential. Ellagic acid treatment was found to induce a reduction in cell number preceded by alterations of the mitochondrial membrane potential and activation of caspase-9 and -3, DNA-fragmentation and cell death by apoptosis. The apoptotic cell death studied was not due to anoikis since it was significant in the adherent fraction of the cells. We conclude that ellagic acid induces dose- and time-dependent apoptosis, at least partly by the mitochondrial pathway, in an embryonal neuronal tumor cell system. This finding is in agreement with previously reported data on adult carcinoma cells thus suggesting a more general effect of ellagic acid on tumor cells.

Place, publisher, year, edition, pages
Elsevier, 2014
National Category
Medical and Health Sciences Pharmacology and Toxicology
Identifiers
urn:nbn:se:kau:diva-29599 (URN)10.1016/j.biopha.2013.08.010 (DOI)000332448400019 ()24051122 (PubMedID)
Available from: 2013-10-17 Created: 2013-10-17 Last updated: 2019-07-09Bibliographically approved
Shu, H., Jönsson, B. A., Larsson, M., Nånberg, E. & Bornehag, C.-G. (2014). PVC flooring at home and development of asthma among young children in Sweden, a 10-year follow-up. Indoor Air, 24(3), 227-235
Open this publication in new window or tab >>PVC flooring at home and development of asthma among young children in Sweden, a 10-year follow-up
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2014 (English)In: Indoor Air, ISSN 0905-6947, E-ISSN 1600-0668, Vol. 24, no 3, p. 227-235Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The incidence of asthma and allergy has increased throughout the developed world over the past decades. During the same period of time the use of industrial chemicals such as phthalates, commonly used as plasticizers in polyvinylchloride (PVC) flooring material, has increased.

AIMS: To investigate if PVC-flooring in the home of children in the age of 1-5 years is associated with the development of asthma in 5-year and 10-year follow-up investigations (n=3,228).

METHODS: Dampness in Buildings and Health Study (DBH Study) commenced in 2000 in Värmland, Sweden. The current analyses included subjects who answered all baseline and follow-up questionnaires. Logistic regression analyses were applied to questionnaire results.

RESULTS: Children who had PVC floorings in the bedroom at baseline were more likely to develop doctor diagnosed asthma during the following 10 years period when compared with children living without. There were indications that PVC flooring in the parents' bedrooms were stronger associated with the new cases of doctor diagnosed asthma when compared with child's bedroom.

CONCLUSIONS: Our results suggest PVC flooring exposure during pregnancy could be a critical period in the development of asthma in children at a later time, prenatal exposure and measurements of phthalate metabolites should be included in the future. This article is protected by copyright. All rights reserved.

Place, publisher, year, edition, pages
John Wiley & Sons, 2014
Keywords
Allergy, Asthma, Children, Dampness in Buildings and Health Study, Endocrine-disrupting chemicals, Incidence, Longitudinal, Phthalates, Polyvinylchloride flooring
National Category
Medical and Health Sciences
Research subject
Public Health Science; Biomedical Sciences
Identifiers
urn:nbn:se:kau:diva-29598 (URN)10.1111/ina.12074 (DOI)000335008300002 ()24118287 (PubMedID)
Available from: 2013-10-17 Created: 2013-10-17 Last updated: 2019-07-10Bibliographically approved
Shu, H., Jönsson, B., Lindh, C., Knutz, M., Nånberg, E., Svensson, Å. & Bornehag, C. G. (2014). PVC flooring in the home is related to urinary levels of phthalates in swedish pregnant women in the selma study. Paper presented at Conference of 13th International Conference on Indoor Air Quality and Climate, Indoor Air 2014 ; Conference Date: 7 July 2014 Through 12 July 2014. Indoor Air, 976-978
Open this publication in new window or tab >>PVC flooring in the home is related to urinary levels of phthalates in swedish pregnant women in the selma study
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2014 (English)In: Indoor Air, ISSN 0905-6947, E-ISSN 1600-0668, p. 976-978Article in journal (Refereed) Published
Place, publisher, year, edition, pages
Elsevier, 2014
Keywords
Endocrine disruptors; Phthalate; Pregnant; PVC; SELMA-Study
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Research subject
Public Health Science
Identifiers
urn:nbn:se:kau:diva-43218 (URN)2-s2.0-84924706203 (Scopus ID)
Conference
Conference of 13th International Conference on Indoor Air Quality and Climate, Indoor Air 2014 ; Conference Date: 7 July 2014 Through 12 July 2014
Available from: 2016-06-30 Created: 2016-06-15 Last updated: 2019-07-10Bibliographically approved
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